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目的 研究 APOE基因多态与血浆连结蛋白 C(PC)的关系。方法 应用聚合酶链反应 -限制性片段多态性 (PCR- RFCP)技术与应用 EL ISA法测定 PC、 Fn35例老年糖尿病 2型患者。对照组为正常健康人 5 0例。结果 糖尿病组以 3/ 4型基因为主 ,健康对照组以 3/ 3型基因分布频率最高。 DM2型、 DM2型并心脑血管病组的等位基因频率分别为 0 .18、 0 .0 3、 0 .79;0 .0 6、 0 .0 6 ,0 .88。糖尿病组基因分布 E3/ 4 >E2 / 3>E3/ 3。PC、Fn与 ε4 呈正相关。结论 APOE4 等位基因携带可能是糖尿病的重要遗传标志。在糖尿病发生、发展中有一定意义
Objective To study the relationship between APOE gene polymorphism and plasma connexin C (PC). Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFCP) and ELISA were used to detect 35 cases of elderly type 2 diabetes mellitus patients with Fn. The control group of normal healthy people 50 cases. Results Type 3/4 gene was predominant in diabetic group, and type 3/3 gene was the highest in healthy control group. The allele frequencies of DM2 type, DM2 type and cardio-cerebrovascular disease group were 0.18, 0.30, 0.79, 0.06, 0.06, 0.88 respectively. Diabetes group gene distribution E3 / 4> E2 / 3> E3 / 3. PC, Fn and ε4 was positively correlated. Conclusion APOE4 allele may be an important genetic marker of diabetes. In diabetes, the development of a certain significance