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采用凝胶过滤、快速液相（FPLC）和高压液相（HPLC）成功地从小牛股骨提取和纯化了骨钙素（BGP）。用其作为抗原免疫家免产生了高效价的抗体，38．5％结合率（Bo／T）的最终稀释度为1：35000。应用此抗体建立的放射免疫分析法，稳定性好，灵敏度高，亲和常数为2．29×1011，回收率为（102±2．2）％，批内变异为2．1％，批间变异为5．6％，同降钙素、甲状旁腺激素和胰岛素无任何交叉反应。81名健康志愿者血清BGP水平为（5．3±1．5）ng／ml。绝经后骨质疏松、原发性甲状旁腺功能亢进症、慢性肾功能不全和畸形性骨炎患者血清BGP值均明显高于健康者。而库欣（Cushing）综合征和甲状旁腺功能减退患者血清BGP水平明显低于健康者。本研究结果表明，骨转换增加的疾病，血BGP值有增高；骨形成降低或者转换减慢的疾病，其血BGP值有下降。因此，血清BGP浓度是反映骨转换敏感而可靠的指标，在骨代谢疾病的临床和科研中具有重要意义。
Osteocalcin (BGP) was successfully extracted and purified from calf bone using gel filtration, fast liquid phase (FPLC) and high pressure liquid phase (HPLC). High-titer antibodies were generated using this antigen as an immunogen, with a final dilution of 1: 35,000 at a 38.5% binding rate (Bo / T). The radioimmunoassay established by this antibody has good stability and high sensitivity, with an affinity constant of 2.29 × 1011, a recovery of (102 ± 2.2)% and an intra-assay variation of 2.1% Variation of 5.6%, with calcitonin, parathyroid hormone and insulin without any cross-reaction. Serum BGP levels in 81 healthy volunteers were (5.3 ± 1.5) ng / ml. Serum BGP values of postmenopausal osteoporosis, primary hyperparathyroidism, chronic renal insufficiency and osteitis of osteomyelitis were significantly higher than those in healthy subjects. Serum BGP levels in patients with Cushing’s syndrome and hypoparathyroidism were significantly lower than those in healthy subjects. The results of this study indicate that diseases with increased bone turnover have elevated blood BGP values, and blood BGP values have decreased in cases of bone formation or slowed conversion. Therefore, serum BGP concentration is sensitive and reliable indicator of bone turnover, which is of great significance in clinical and research of bone metabolism diseases.