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目的探讨磷酸受纳蛋白(PLB)小干扰RNA对感染性休克大鼠左心功能的影响。方法雌性Wistar大鼠75只,随机分为假手术组、感染性休克组、盐水组、PLB siRNA组和对照组,每组15只,通过腺病毒表达载体构建重组RNA干扰腺病毒表达载体(pAdeno-X-siPLB),并导入大鼠心肌,6周后,测量左室舒张末期压(LVEDP),左室收缩压(LVSP),左室内压最大上升和下降速率(±dP/dtmax),RT-PCR和Western-blotting分别检测PLB表达水平。结果感染性休克组、盐水组PLB mRNA表达水平显著高于对照组(P<0.05);PLB siRNA组PLB mRNA表达水平显著低于感染性休克组(P<0.05);感染性休克组、盐水组PLB mRNA表达水平比较差异无统计学意义;感染性休克组、盐水组和PLB siRNA组SR Ca~(2+)-ATPase活力显著低于对照组(P<0.05);PLB siRNA组PLB SR Ca~(2+)-ATPase活力显著高于感染性休克组(P<0.05);感染性休克组、盐水组SR Ca~(2+)-ATPase活力比较差异无统计学意义;感染性休克组、盐水组、PLB siRNA组LVSP、+dP/dtmax、-dP/dtmax显著低于对照组(P<0.05),LVEDP显著高于对照组(P<0.05);PLB siRNA组LVSP、+dP/dtmax、-dP/dtmax显著高于感染性休克组(P<0.05),LVEDP显著低于感染性休克组(P<0.05);感染性休克组、盐水组LVSP、LVEDP、+dP/dtmax、-dP/dtmax比较差异无统计学意义。结论通过构建PLB小干扰RNA,心肌注射后对PLB表达有一定的沉默作用,从而提高SR Ca~(2+)-ATPase活性,提高心脏储备,改善心功能。
Objective To investigate the effect of phosphorylated small molecule RNA (PLB) RNA on left ventricular function in septic shock rats. Methods Seventy five female Wistar rats were randomly divided into sham operation group, septic shock group, saline group, PLB siRNA group and control group, with 15 rats in each group. The recombinant adenovirus vector was constructed by adenovirus expression vector (pAdeno -X-siPLB) were injected into the myocardium of rats. After 6 weeks, the left ventricular end-diastolic pressure (LVEDP), left ventricular systolic pressure (LVSP) and maximum ± dP / dtmax -PCR and Western-blotting were used to detect the expression of PLB. Results The expression of PLB mRNA in septic shock group and saline group was significantly higher than that in control group (P <0.05). PLB mRNA expression in PLB siRNA group was significantly lower than that in septic shock group (P <0.05) (P <0.05). The PLB SR Ca ~ (2 +) - ATPase activity in PLB siRNA group was significantly lower than that in control group (2 +) - ATPase activity in septic shock group was significantly higher than that in septic shock group (P <0.05). There was no significant difference in SR Ca ~ (2 +) - ATPase activity between septic shock group and saline group LVP, + dP / dtmax and -dP / dtmax in PLB siRNA group were significantly lower than those in control group (P <0.05), LVEDP was significantly higher than that in control group (P <0.05) dP / dtmax was significantly higher than that of septic shock group (P <0.05), LVEDP was significantly lower than that of septic shock group (P <0.05); LVSP, LVEDP, + dP / dtmax and -dP / dtmax The difference was not statistically significant. Conclusion The expression of SR Ca ~ (2 +) - ATPase can be increased by enhancing the activity of SR Ca ~ (2 +) - ATPase, improving cardiac reserve and improving heart function by constructing PLB small interfering RNA.