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为了比较玉米(ZeamaysL.)(C4)与小麦(TriticumaestivumL.)(C3)黑暗向光照变化过程中的光诱导适应性反应,对非光化学猝灭(Nonphotochemicalquenching,qN)的动态变化特征进行了观察和分析。结果表明,在15min至24h的不同暗适应处理中,根据qN的光适应的动态变化可分为F型(快速稳定型)、M型(中速稳定型)和S型(慢速稳定型)3种。在暗适应后的光诱导期间,小麦qN主要表现出M和F型,玉米则表现出S和M型。通过对主要的荧光参数分析,小麦与玉米的Fv/Fm,qP和ΦPSⅡ差异较小,而qN稳定值有一定的差异,但TqNmax(qN达到最大值的时间)和TqN(qN达到稳定值的时间)玉米明显高于小麦,差异极明显。进一步分析经黑暗处理后光诱导的光合速率和气孔导度的动态变化,两作物虽然在增加的速率和稳定后的绝对值有差异,但达到稳定的时间无明显的不同。通过塑料膜包封叶片的方法阻止CO2同化,而qN变化特征也基本不改变。这意味着短时间的C代谢不明显影响qN对光诱导的反应。对光系统Ⅰ反应中心P700的典型氧化还原方式的测定和电子库容能力的分析也表明它们不是造成qN动态变化的主要原因。然而,对qNmax成分的分析证明高能态猝灭(highenergystatequenching,qE)、转化猝灭(transitionquenching,qT)和光抑制猝灭(photoinhibitoryquenching,qI)分别为55.6%、18.5%和25.9%,说明qE是导致玉米qN在暗处理后的光诱导表现出TqNmax高、TqN长的主要原因。这意味着玉米(C4)在暗—光的变化中维持较高的qE,保持较高的类囊体质子梯度,有益于启动NADPH和ATP的形成。
In order to compare the light-induced adaptive responses of dark-to-light changes of maize (Zea mays L.) (C4) and Triticum aestivum L. (C3), the dynamic changes of non-photochemical quenching (qN) analysis. The results showed that the dynamic changes of light adaptation according to qN can be divided into F type (fast stable type), M type (medium stable type) and S type (slow stable type) in different dark adaptation treatments from 15min to 24h. 3 types. During dark-adapted light-induced period, wheat qN mainly showed M and F types, while maize showed S and M types. Fv / Fm, qP and ΦPSⅡhad little difference between wheat and maize, but there were some differences in qNstability between wheat and maize. However, TqNmax (time when qN reached the maximum) and TqN Time) corn is significantly higher than wheat, the difference is obvious. Further analysis of the photosynthetic rate and stomatal conductance induced by darkness showed that there was no significant difference between the two crops in terms of increasing rate and absolute value after stabilization. The method of encapsulating the leaf with a plastic film prevents CO2 assimilation, while the change of qN does not change basically. This means that short-term C metabolism does not significantly affect the qN-induced response. The determination of the typical redox mode and the analysis of the electronic capacity of P700 in the photo-system I reaction center also showed that they are not the main reason for the dynamic change of qN. However, analysis of the qNmax components demonstrated that the highenergy quenching (qE), transition quenching (qT) and photoinhibitoryquenching (qI) were 55.6%, 18.5% and 25.9%, respectively, indicating that qE is the Light induction of maize qN after dark treatment showed the main reason for the high TqNmax and long TqN. This means that corn (C4) maintains a high qE in the dark-light changes, maintains a high protoplast gradient and promotes the formation of NADPH and ATP.