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PHR1是一种转录因子,在植物对磷的吸收过程中起重要作用,本研究为了获得高磷利用效率的小麦,采用基因枪法遗传转化了普通小麦品种矮抗58,获得转缺磷响应转录因子Ta PHR1基因小麦新品系T8,PCR和RT-PCR检测表明外源基因已经整合到小麦基因组并正确转录,为明确该转基因小麦的磷利用特性,以T8和其受体对照矮抗58为试验材料,研究了试材的地上部吸磷量和产量性状。结果表明,转Ta PHR1基因小麦新品系T8在CK、N、NK和NPK处理下的产量和地上部吸磷量均显著高于其受体对照矮抗58,其中在N处理下的产量可增加10.64%,吸磷量可增加12.29%;大田条件下转基因小麦单株产量和小区产量分别较其受体矮抗58增产10.49%和7.32%,千粒重的增加是T8增产的主要因素;转Ta PHR1基因小麦较对照具有更好的磷吸收特性和增产潜力。
PHR1 is a transcription factor that plays an important role in plant uptake of phosphorus. In order to obtain high phosphorus use efficiency, we studied the genetic transformation of common wheat variety Dwarf Anti-58 using the gene gun method, T8, PCR and RT-PCR of Ta PHR1 wheat lines showed that exogenous genes had been integrated into the wheat genome and correctly transcribed. To determine the phosphorus utilization characteristics of the transgenic wheat, T8 and its receptor control A58 , The phosphorus uptake and yield characteristics of shoots were studied. The results showed that the translocation of P uptake and P uptake by Ta-PHR1 transgenic wheat line T8 under CK, N, NK and NPK treatments were significantly higher than that of its receptor-treated shoot line A58, and its yield under N treatment increased 10.64% and P uptake 12.29% respectively. The yield per plant and yield of transgenic wheat increased 10.49% and 7.32%, respectively, compared with its receptor A58. The increase of 1000-grain weight was the main factor of T8 yield increase. Compared with the control, the transgenic wheat had better phosphorus absorption and yield potential.