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四、瓶苗培养将接种好的三角瓶,放入无菌箱内或窗台上散射光处。无菌箱内用日光灯补光,温度保持18～29℃都能正常生长,最适温是20～25℃。顺便说一下,剪切叶片时,要选生长健壮、不太老也不太嫩的叶片,接种时期最好避开炎夏。外植体经过一个多月的培养,开始叶片弯曲,部分部位变厚——都是细胞分裂增殖的征兆。不久,外植体上可长出分生组织或直接长出许多不定芽。40多天后,可根据生长情况分瓶。用手术刀对外植体进行切割,植入原配方的培养基中继代培
Fourth, bottle seedlings will be vaccinated a good flask, into the sterile box or windowsill scattering light. Aseptic tank with fluorescent light, the temperature maintained at 18 ~ 29 ℃ can grow normally, the optimum temperature is 20 ~ 25 ℃. By the way, when cutting the leaves, it is better to choose the leaves that grow stronger, not too old and not too tender. After more than a month of explant culture, the leaf starts to bend and thickens in some areas - both as signs of cell division and proliferation. Soon, explants may grow meristems or grow adventitious buds directly. More than 40 days, according to the growth of the bottle. The explant was cut with a scalpel and implanted into the original formulation of medium for subculture