本文报告了纯化皂苷佐剂QS-21对产生抗大肠杆菌O多糖（PS)、PS结合菌苗以及脱毒脂多糖（LPS）抗体应答的增强作用。 以热酚抽提法从大肠杆菌O18培养物中提取LPS;经酸水解获得PS;用碱处理纯化的LPS得脱毒LPS（dLPS）PS与白喉类毒素结合成PS-白喉类毒素结合菌苗（PS-DT）。分别将游离PS,PS-DT与dLPS单独或与QS-21联合接种小鼠,以检测其免疫原 This article reports the enhanced effect of purified saponin adjuvant QS-21 on the production of antibodies against E. coli O polysaccharide (PS), PS-conjugated bacterins and detoxified lipopolysaccharide (LPS). LPS was extracted from E. coli O18 culture by hot phenol extraction; PS was obtained by acid hydrolysis; and purified LPS was treated with alkali to detoxify LPS (dLPS) PS and diphtheria toxoid to form PS-diphtheria toxoid-conjugated vaccine (PS-DT). Free PS, PS-DT and dLPS, alone or in combination with QS-21, were inoculated with mice to detect their immunogen