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目的：探讨细胞因子ＧＭ－ＣＳＦ、ＩＬ－８、ＴＮＦ－α在哮喘发病中的作用及雷公藤对细胞因子产生的影响。方法：以卵蛋白雾化吸入诱导哮喘豚鼠实验模型，用雷公藤处理，斑点印迹杂交检测支气管肺组织ＧＭ－ＣＳＦｍＲＮＡ表达，ＥＬＩＳＡ测定血浆中ＩＬ－８、ＴＮＦ－α含量。结果：哮喘组豚鼠肺组织ＧＭ－ＣＳＦｍＲＮＡ表达和血浆中ＴＮＦ－α水平明显增高，但血浆中ＩＬ－８升高不明显。雷公藤处理后ＧＭ－ＣＳＦｍＲＮＡ表达及ＴＮＦ－α水平降低。结论：ＧＭ－ＣＳＦ等细胞因子产生增加，可能是导致哮喘气道炎症的原因。雷公藤能抑制细胞因子的产生，可能在哮喘患者抗炎治疗中有潜在的应用价值
Objective: To investigate the role of cytokines GM-CSF, IL-8 and TNF-α in the pathogenesis of asthma and the effect of triptolide on cytokine production. Methods: Asthmatic guinea pigs were induced by nebulized ovalbumin, and the expression of GM-CSF mRNA in bronchial lung tissue was detected by tripterygium wilfordii and dot blot hybridization. The levels of IL-8 and TNF-α in plasma were measured by ELISA. Results: The expression of GM-CSF mRNA and the level of TNF-α in plasma of asthmatic guinea pigs were significantly increased, but the levels of IL-8 in plasma were not significantly increased. After treatment of Tripterygium wilfordii, GM-CSF mRNA expression and TNF-α level decreased. Conclusion: Increased production of cytokines such as GM-CSF may be the reason of airway inflammation in asthma. Tripterygium can inhibit the production of cytokines, which may have potential value in anti-inflammatory treatment of asthma patients