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目的 探讨人类白细胞抗原 (HL A) - B5 1与 Behcet ) 病葡萄膜炎间的相关性。 方法 应用微量淋巴细胞毒试验 ,对 2 7例 Behcet ) 病患者和 30例健康人的 HL A- A和 HL A- B抗原进行研究。应用聚合酶链式反应 -序列特异性引物 (PCR- SSP)技术测定 HL A- B5 1抗原阳性的 Behcet ) 病患者和对照组人群中的HL A- B5 1等位基因 (HL A- B* 5 10 1- B* 5 10 7)。 结果 Behcet ) 病患者和对照组 HL A- B5 1的阳性频率分别为 6 3%和 16 .7% [χ2 =12 .9,P<0 .0 0 1,Pc<0 .0 5 ,相对危险度 (RR) =8.5 ]。在 Behcet ) 病患者中 ,葡萄膜炎的患病率为 4 0 .7% (11/ 2 7) ;72 .7% (8/ 11) Behcet ) 病葡萄膜炎中存在 HL A- B5 1(均为 HL A- B* 5 10 1)。HL A- B5 1和 HL A- B* 5 10 1与 Behcet ) 病葡萄膜炎存在弱相关性 ,但均未见统计学差异 (分别为 RR=2 .0 7,χ2 =0 .75 9,P >0 .2 5 ;RR =2 .6 7,χ2 =1.395 ,P >0 .10 )。 结论 提示 HL A- B5 1(HL A- B* 5 10 1)是Behcet ) 病的易感基因 ,并与葡萄膜炎有一定的相关性
Objective To investigate the correlation between human leukocyte antigen (HL A) - B5 1 and Behcet’s disease uveitis. Methods Twenty-seven patients with Behcet’s disease and 30 healthy subjects were studied with HL-A and HL A-B antigens by means of micro-cytotoxicity test. The HL-B5 1 allele (HL-B * 1) in patients with HL-B5 1 antigen-positive and control subjects was detected by polymerase chain reaction-sequence specific primers (PCR-SSP) 5 10 1- B * 5 10 7). Results The positive frequencies of HL-B5 1 in patients with Behcet disease and controls were 63% and 16.7%, respectively (χ2 = 12.9, P <0.01, Pc <0.05, relative risk Degree (RR) = 8.5]. In patients with Behcet’s disease, the prevalence of uveitis was 40.7% (11/27); 72.7% (8/11) of patients had uveitis with HL-B5 1 All HL A- B * 5 10 1). HL-B5 1 and HL-B * 5 10 1 had a weak correlation with Behcet’s disease uveitis, but no statistical difference was found (RR = 2.07, χ2 = 0.759, P> 0 .25; RR = 2.67, χ2 = 1.395, P> 0 .10). Conclusions HL-B5 1 (HL A- B * 5 10 1) is a susceptibility gene for Behcet’s disease and has some correlation with uveitis