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目的:建立注射用硫酸头孢匹罗的HPLC含量测定方法。方法:采用[SHIMADZU VP-ODS C_(18)(150mm×4.6mm,5μm)色谱柱];流动相:0.02mol·L~(-1)磷酸二氢铵溶液[用磷酸溶液(1→10)调节pH 3.3]-乙腈(90:10);检测波长:265nm;柱温:25℃;流速:1.0ml·min~(-1)。结果:头孢匹罗在47.79～189.56μg·ml~(-1)浓度范围呈良好的线性关系,r=0.9999;平均回收率为99.2%,RSD=1.2%(n=9)。结论:本法简便快捷,准确可靠,重复性好。
Objective: To establish a HPLC method for the determination of cefpirome sulfate for injection. The mobile phase consisted of 0.02mol·L -1 ammonium dihydrogen phosphate solution [treated with phosphoric acid solution (1 → 10) Adjusted to pH 3.3] -acetonitrile (90:10); detection wavelength: 265 nm; column temperature: 25 ° C .; flow rate: 1.0 ml · min -1. Results: The linear range of cefpirome was 47.79 ~ 189.56μg · ml ~ (-1), r = 0.9999. The average recovery was 99.2% with RSD = 1.2% (n = 9). Conclusion: This method is simple, accurate, reliable and reproducible.