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目的:通过将不同粒径和剂量的放射性纳米示踪剂,注射于兔声带中,观察其在喉前哨淋巴结(SLN)活检中的作用,为喉癌SLN活检选择较佳粒径和剂量的放射性纳米示踪剂提供实验依据。方法:将30只新西兰成年雄兔,随机分为6组,每组5只新西兰兔。分别将50nm～0.01ml,50nm～0.02ml,80nm～0.01ml,80nm～0.02ml,100nm～0.01ml,100nm～0.02ml的99mTc硫胶体注入兔声带中,观察每组实验检出的SLN的枚数,SLN开始具有放射性的时间,SLN放射性最强的时间以及SLN的放射性持续时间。结果:6组实验中检出的SLN在1～3枚之间,共45枚,分布在Ⅱ区,Ⅲ区和Ⅳ区,其中50nm～0.02ml组检出的SLN的枚数最多,100nm～0.01ml组检出的SLN的枚数最少,两者之间差异有统计学意义(P<0.05)。6组实验中,SLN开始具有放射性和具有最强放射性的时间出现最早的均为50nm～0.02ml组,分别为49.20s和178.60s;而开始具有放射性和具有最强放射性的时间出现最晚的均为100nm～0.01ml组,分别为235.80s和311.20s。各组在30min内均可持续观察到SLN的放射活性。结论:50nm～0.02ml注射时,99mTc硫胶体在淋巴管中移动速度较快,淋巴结摄取率高,并且SLN的放射性可以持续至少30min,为喉SLN活检应用较佳的粒径和剂量。
OBJECTIVE: To observe the role of radioactive nano-tracer in rabbit’s vocal cords in SLN biopsy and to choose the best particle size and dose for SLN biopsy of laryngeal carcinoma Nanotracer provides experimental evidence. Methods: Thirty New Zealand adult male rabbits were randomly divided into 6 groups of 5 New Zealand rabbits. 99mTc sulfur colloids of 50nm ~ 0.01ml, 50nm ~ 0.02ml, 80nm ~ 0.01ml, 80nm ~ 0.02ml, 100nm ~ 0.01ml, 100nm ~ 0.02ml were injected into the rabbit vocal cords respectively. The number of SLNs , The time at which SLN begins to have radioactivity, the time when SLN is the most radioactive, and the duration of radioactivity of SLN. Results: SLNs detected in 6 groups were between 1 and 3, with a total of 45 SLNs distributed in Zone II, Zone III and Zone IV, among which the number of SLN detected in the range of 50 nm to 0.02 ml was the highest, ranging from 100 nm to 0.01 ml group detected the least number of SLN, the difference between the two was statistically significant (P <0.05). In the six experiments, the earliest time for SLN to start to have radioactivity and the strongest radioactivity appeared in the group of 50nm-0.02ml, which was 49.20s and 178.60s, respectively; while the time with the highest radioactivity and the most intense radioactivity appeared the latest All were 100nm ~ 0.01ml group, respectively 235.80s and 311.20s. The activity of SLN was continuously observed in all groups within 30 min. CONCLUSIONS: 99mTc sulfur colloids migrate faster in lymphatic vessels and have a higher rate of lymphatic uptake at 50nm ~ 0.02ml injection, and the radioactivity of SLN can persist for at least 30min, which is the optimal particle size and dose for laryngeal SLN biopsy.