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利用ＢＣＧ激活的外周血单核细胞（ＰＢＭＮＣ）作为肿瘤杀伤细胞———ＢＡＫ细胞，观察其对移行上皮肿瘤细胞系ＢＴＡ和１１例原代培养细胞的细胞毒作用。结果表明，单独ＢＣＧ对培养肿瘤细胞没有细胞毒作用；ＮＫ细胞仅表现较低的细胞毒作用，在效靶比４０∶１对ＢＴＡ的特异性杀伤率为１７．６３％，对原代细胞为１２．４９％；ＢＡＫ细胞分别为３１．２６％和１９．１６％（Ｐ＜０．０５）；ＬＡＫ细胞分别为３５．５３％和２４．２１％；对ＢＴＡ细胞的细胞毒作用ＬＡＫ细胞＞ＢＡＫ细胞（Ｐ＜０．０５），对原代细胞的细胞毒作用ＬＡＫ细胞和ＢＡＫ细胞之间无显著性差异（Ｐ＞０．０５）。认为ＢＡＫ细胞是不同于ＬＡＫ细胞的一类肿瘤杀伤细胞，在ＢＣＧ抗肿瘤的过程中起着重要作用。
BCG-activated peripheral blood mononuclear cells (PBMNC) were used as tumor killer cells - BAK cells to observe the cytotoxic effect on the transplanted epithelial tumor cell line BTA and 11 primary cultured cells. The results showed that BCG alone had no cytotoxic effect on the cultured tumor cells; NK cells showed only low cytotoxicity, specific killing rate of BTA was 17.63% BAK cells were 31.26% and 19.16% (P <0.05), LAK cells were 35.53% and 24.21%, respectively. The cytotoxicity against BTA cells The effect of LAK cells> BAK cells (P <0.05) had no significant difference on the cytotoxicity of primary cells between LAK cells and BAK cells (P> 0.05). BAK cells are considered to be a type of tumor killer cell that is distinct from LAK cells and play an important role in the anti-tumor effect of BCG.