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目的采用体外消化/Caco-2细胞模型评价3种生物强化玉米铁生物利用率,筛选出铁生物利用率高的玉米品种。方法将高铁玉米品种(中铁2号、中铁3号、中铁4号)和低铁玉米品种(正大818、正大619)磨粉,测定玉米粉中铁含量,经体外消化后进行Caco-2细胞吸收实验,以细胞单位质量蛋白质中铁蛋白含量作为铁生物利用率的指标,并计算单位质量玉米粉铁生物利用率。同时测定玉米粉总磷含量,以此反映玉米中植酸水平。结果中铁2号玉米粉中铁含量(33.25mg/kg)、铁生物利用率和单位质量玉米粉铁生物利用率最高,而总磷含量较低;铁生物利用率其次为中铁3号和正大818,正大619玉米粉最低。结论中铁2号玉米品种的铁含量与生物的利用率最高,可推荐为后续人群试验试用品种。
OBJECTIVE: To evaluate the bioavailability of three kinds of bio-enhanced maize iron by in vitro digestion / Caco-2 cell model and to screen maize varieties with high bioavailability of iron. Methods High-speed rail corn varieties (Zhongtie 2, Zhongtie 3 and Zhongtie 4) and low-iron maize varieties (Zhengda 818 and Zhengda 619) were ground to determine the content of iron in corn meal. After in vitro digestion, Caco-2 cells were harvested , Using the ferritin content in the protein as the index of iron bioavailability and calculating the bioavailability of iron and iron per unit mass of corn. Determination of total phosphorus content of corn meal, in order to reflect the level of phytic acid in corn. Results The iron content (33.25mg / kg), the bioavailability of iron and the quality of iron and iron per unit mass of corn in China Railway No. 2 were the highest, while the content of total phosphorus was lower. The bioavailability of iron was the second highest in Zhongtie No.3 and Zhengda 818, Chia Tai 619 corn flour minimum. Conclusion The iron content and bioavailability of China Railway No. 2 maize varieties are the highest, which can be recommended for subsequent trial population trials.