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差异显示逆转录ＰＣＲ（ＤＤＲＴ－ＰＣＲ）技术是一种筛选和克隆差异表达基因的新方法。该方法利用ｃＤＮＡ逆转录技术、ＰＣＲ（ｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ）的高效扩增和凝胶电泳分离技术，能同时显示多种来自相关细胞的ｍＲＮＡ样品。可用于多种研究目的，如鉴别和观察基因表达的改变；差异表达基因的迅速测序并与基因库比较；单个的差异表达基因片段能迅速克隆并制成探针从ｃＤＮＡ文库或基因组文库中分离基因等。ＤＤＲＴ－ＰＣＲ技术具有迅速、简便、灵敏和高效等优点，已被广泛地应用于癌症、心脏疾病、细胞分化、衰老及其它领域的研究中，并且在应用中被不断地改进和发展而更趋合理和完善。
Differential Display Reverse transcription PCR (DDRT-PCR) is a novel method for screening and cloning differentially expressed genes. The method utilizes cDNA reverse transcription technology, efficient amplification of PCR (polymerase chain reaction) and gel electrophoresis separation technology to simultaneously display a plurality of mRNA samples from related cells. Can be used for a variety of research purposes such as identifying and observing changes in gene expression; rapid sequencing of differentially expressed genes and comparison with gene banks; single differentially expressed gene fragments can be rapidly cloned and made into probes for isolation from cDNA libraries or genomic libraries Genes and so on. The rapid, simple, sensitive and efficient DDRT-PCR technology has been widely used in cancer, heart disease, cell differentiation, aging and other areas of research, and in the application of continuous improvement and development and more Reasonable and perfect.