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建立了ＨＰＬＣ法同时测定家犬血浆中的醋氯芬酸及其主要代谢物的浓度。此法简便易行，精密度好，方法回收率９１．３％～９６．９％，日内、日间ＲＳＤ为３．６９％～８．１３％，血药浓度在０．０５０～５１．２μｇ·ｍｌ－１范围内呈线性关系，相关系数０．９９９８，当Ｓ／Ｎ≥３时，最小检测浓度为１０ｎｇ·ｍｌ－１。此法可同时测定醋氯芬酸及其在体内的主要代谢物。醋氯芬酸ｐｏ吸收迅速，给药后约１２ｍｉｎ即达血药浓度峰值，其药—时曲线符合二室模型，Ｔ１／２α仅为２．５ｍｉｎ左右，Ｔ１／２β约为１３７ｍｉｎ，代谢物约在１１０ｍｉｎ达到血药浓度峰值，峰浓度为３．２０μｇ·ｍｌ－１，Ｔ１／２β约为１４０ｍｉｎ。
A HPLC method was established for the simultaneous determination of aceclofenac and its major metabolites in dog plasma. The method is simple and easy to operate with good precision. The method recoveries ranged from 91.3% to 96.9%. The intra-day and inter-day RSD were 3.69% ~ 8.13% and the plasma concentrations were 0.050 ~ 51.2μg · Ml-1, the correlation coefficient was 0.9998. When S / N≥3, the minimum detectable concentration was 10 ng · ml-1. This method allows the simultaneous determination of aceclofenac and its major metabolites in the body. Aceclofenac po absorbed quickly, about 12min after administration reached the peak plasma concentration, the drug-time curve in line with the two-compartment model, T1 / 2α only about 2.5min, T1 / 2β about 137min, metabolites about At 110min to reach the plasma concentration peak, the peak concentration of 3.20μg · ml-1, T1 / 2β about 140min.