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目的:探讨人巨细胞病毒(human cytomegalov-irus,HCMV)UL147基因在婴儿巨细胞病毒肝炎临床株中的多态性及其与致病性之间的关系.方法:对25株经荧光定量PCR方法检测HCMVDNA为阳性的临床株的尿液上清液进行HCMVUL147全序列PCR扩增,并对PCR扩增产物进行序列测定及分析.结果:25株临床株扩增阳性产物进行HCMVUL147全序列PCR扩增,结果有19株PCR扩增阳性;与HCMVToled株进行序列比较分析,所有UL147开放读码框架(open-reading-fraem,ORF)长度在477-480bp,序列呈现较高多态性,UL147基因变异率为2.7%-10.9%;氨基酸变异集中在30-40氨基酸位点,变异率为4.3%-8.75%;所有研究标本中没有序列与Toledo完全一致.序列差异多位于序列的5’端,巨细胞病毒肝炎患儿和无症状患儿之间的UL147基因DNA序列是一致的.NCBIPROS数据库预测UL147编码蛋白功能区显示几乎所有的UL147序列中都存在1个CKP和1个PKC位点.结论:巨细胞病毒肝炎患儿临床株中的HCMVUL147基因序列呈现高度多态性.UL147基因多态性与其对肝脏损害的程度无关.
Objective: To investigate the polymorphism of human cytomegalov-irus (HCMV) UL147 gene in clinical isolates of infant cytomegalovirus hepatitis and its relationship with pathogenicity.Methods: Methods HCMVUL147 full-length PCR was performed on the urine supernatant of HCMV DNA positive clinical isolates, and the PCR amplification products were sequenced and analyzed.Results: The positive products of 25 clinical isolates were amplified by HCMV UL147 PCR The results showed that 19 of them were positive for PCR amplification. Compared with HCMVToled, the sequence of open reading-fraem (ORF) of UL147 was 477-480bp in length and the sequence was highly polymorphic. The UL147 gene The mutation rates ranged from 2.7% to 10.9%, and the amino acid variations concentrated on the 30-40 amino acid sites with a mutation rate of 4.3% to 8.75%. No sequence was completely consistent with Toledo in all the studied samples. The UL147 DNA sequence was identical between children with cytomegalovirus hepatitis and asymptomatic children.NCBIPROS database predicted that the UL147-encoded protein functional region showed one CKP and one PKC site in almost all UL147 sequences. : Cytomegalovirus liver HCMVUL147 gene sequences in children with clinical presentation strains highly polymorphic .UL147 unrelated gene polymorphism and the degree of liver damage.