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CALC Ⅰ基因最初的转录通过替代切接产生两个不同的mRNA。由降低钙素mRNA编码的一种蛋白质前体含有一个氨基末端肽、CT和一个21个氨基酸CT羧基末端肽(CCP Ⅰ)。而CGRP编码的前体含有相同氨基末端肽和CGRP。近来在人甲状腺髓样癌(MTC)证实,还另有一替代途径,产生一种新的CT mRNA,并在正常人甲状腺也有很少量。这一转录由外显子1、2、3、部分外显子4、外显子5和多聚核苷酸外显子6组成,含有一编码为已知氨基末端肽和CT的开放阅读框架和新的羧基末端肽CCPⅡ。CCPⅡ包括21个氨基酸,在基羧基末端的8个氨基酸不同于CCPⅠ,本文用快速特异的单克隆抗体(MAb)免疫荧光方法显示CCPⅡ,双重标记的免疫荧光显示CCPⅡ/CT,CCPⅡ/CGRP。
The initial transcription of the CALC I gene produces two different mRNAs by alternative cleavage. One of the protein precursors encoded by the reduced calcitonin mRNA contains an amino terminal peptide, CT, and a 21 amino acid CT carboxy terminal peptide (CCP I). While CGRP-encoded precursors contain the same amino terminal peptide and CGRP. Recently in human medullary thyroid carcinoma (MTC) confirmed that there is another alternative pathway to produce a new CT mRNA, and also in normal human thyroid a very small amount. This transcription consists of exons 1, 2 and 3, partial exon 4, exon 5 and polynucleotide exon 6 and contains an open reading frame encoding a known amino terminal peptide and CT And the new carboxyl terminal peptide CCPII. CCP Ⅱ contains 21 amino acids, and the 8 amino acids at the carboxyl end of the carboxyl group are different from CCPⅠ. CCP Ⅱ was detected by fast specific monoclonal antibody (MAb) immunofluorescence and CCP Ⅱ / CT and CCPⅡ / CGRP by double labeled immunofluorescence.