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观察角质细胞生长因子对放疗和环磷酰胺引起胸腺萎缩的增殖作用。取正常BALB/C雄性小鼠,随机分为KGF处理组和对照组。KGF处理组和对照组均先给予4.5Gy钴射线照射,24h后分别连续皮下注射KGF(5mg/kg.d)和生理盐水7d。4周检测小鼠胸腺和脾脏组织的总细胞数和各T细胞亚群的细胞数;取正常BALB/C雄性小鼠,随机分为KGF处理组和对照组。KGF处理组和对照组均先连续腹腔注射环磷酰胺(300mg/kg)3d,注射完毕24h后再分别连续皮下注射KGF(5mg/kg·d)和生理盐水7d。2周后检测小鼠胸腺和脾脏组织的总细胞数和各T细胞亚群的细胞数。结果:细胞计数和流式细胞仪检测显示,分别经放疗和环磷酰胺处理的小鼠,与对照组相比,KGF处理组平均胸腺细胞总数和CD4SP、CD8SP、DP、DN各T细胞亚群的平均细胞数以及KGF处理组平均脾脏细胞总数和CD4SP、CD8SP各T细胞亚群的平均细胞数均有明显增加(P<0.05)。角质细胞生长因子对放疗和环磷酰胺引起的胸腺萎缩均具有增殖作用,同时可以增加成熟T细胞向脾脏的输出而增强机体的免疫功能。
Observe the proliferation of keratinocyte atrophy induced by radiotherapy and cyclophosphamide. Normal BALB/C male mice were randomly divided into KGF treatment group and control group. Both KGF-treated group and control group were first given 4.5Gy cobalt radiation, 24h after continuous subcutaneous injection of KGF (5mg/kg.d) and saline 7d. The total number of thymus and spleen tissues and the number of cells in each T cell subset were measured at 4 weeks. Normal BALB/C male mice were randomly divided into KGF treated group and control group. Both KGF-treated and control groups received continuous intraperitoneal injection of cyclophosphamide (300 mg/kg) for 3 days. 24 hours after the injection, KGF (5 mg/kg·d) and normal saline were administered subcutaneously for 7 days. After 2 weeks, the total number of cells in the thymus and spleen tissues and the number of cells in each T cell subset were measured. RESULTS: Cell counts and flow cytometry showed that the total number of thymocytes and CD4SP, CD8SP, DP, and DN subgroups of T cells in the KGF-treated group were higher in the mice treated with radiotherapy and cyclophosphamide than in the control group. The average number of cells, the average number of spleen cells in the KGF-treated group, and the average cell number of the CD4SP and CD8SP T cell subpopulations all significantly increased (P<0.05). Keratinocyte growth factor has a proliferative effect on radiotherapy and cyclophosphamide-induced thymus atrophy, and can increase the output of mature T cells to the spleen and enhance the body’s immune function.