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目的建立HPLC波长切换联合梯度洗脱法同时测定愈肝片中獐牙菜苦苷、芒果苷、异荭草苷、当药醇苷、金丝桃苷、异槲皮苷、4,5-O-二咖啡酰奎宁酸和异鼠李素-3-O-葡萄糖苷的含量。方法采用Kromasil C18(250 mm×4.6 mm,5.0μm),以乙腈(A)-0.04%磷酸溶液(B)为流动相,进行梯度洗脱,流速0.9 mL·min~(-1),柱温30℃,进样量为10μL。獐牙菜苦苷、芒果苷、异荭草苷和当药醇苷检测波长为254 nm,金丝桃苷、异槲皮苷、4,5-O-二咖啡酰奎宁酸和异鼠李素-3-O-葡萄糖苷检测波长为345 nm。结果獐牙菜苦苷、芒果苷、异荭草苷、当药醇苷、金丝桃苷、异槲皮苷、4,5-O-二咖啡酰奎宁酸和异鼠李素-3-O-葡萄糖苷8种成分的质量浓度分别在10.55~211.0 mg·L~(-1)(r=0.999 8),14.76~295.2 mg·L~(-1)(r=0.999 9),6.790~135.8 mg·L~(-1)(r=0.999 3),9.080~181.6 mg·L~(-1)(r=0.999 7),4.360~87.20 mg·L~(-1)(r=0.999 9),2.320~46.40 mg·L~(-1)(r=0.999 9),13.69~273.8 mg·L~(-1)(r=0.999 5),2.350~47.00 mg·L~(-1)(r=0.999 4)内线性关系良好;平均加样回收率分别为97.97%、99.88%、98.79%、97.12%、98.21%、100.02%、98.96%和99.35%,RSD分别为1.49%、0.81%、1.10%、0.84%、1.61%、0.78%、1.79%和1.52%。结论所建立的HPLC波长切换联合梯度洗脱法同时测定愈肝片中8种成分,该方法灵敏度高、准确度高、专属性好,为愈肝片的质量控制提供依据。
OBJECTIVE To establish simultaneous determination of swertia glycoside, mangiferin, isoorientin, cortex glycosides, hyperoside, isoquercetin, 4,5-O - Dicaffeoylquinic acid and isorhamnetin-3-O-glucoside content. Methods The mobile phase consisted of Kromasil C18 (250 mm × 4.6 mm, 5.0 μm) and acetonitrile (A) -0.04% phosphoric acid solution (B) with gradient elution at a flow rate of 0.9 mL · min -1 .The column temperature 30 ℃, injection volume of 10μL. Swertia glycoside, mangiferin, isoorientin and when the drug glycosides detection wavelength of 254 nm, hyperoside, isoquercetin, 4,5-O-dicaffeoylquinic acid and isomer Lee Su-3-O-glucoside detection wavelength of 345 nm. Results Swerterin glycosides, mangiferin, isoorientin, when the drug glycosides, hyperoside, isoquercetin, 4,5-O-dicaffeoylquinic acid and isorhamnetin-3- The results showed that the mass concentration of the eight components of O-glucoside were 10.55-211.0 mg · L -1 (r = 0.999 8), 14.76-295.2 mg · L -1 (r = 0.999 9), 6.790 ~ (R = 0.999 3), 9.080 ~ 181.6 mg · L -1 (r = 0.999 7), 4.360 ~ 87.20 mg · L -1 (r = 0.999 9) ), 2.320 ~ 46.40 mg · L -1 (r = 0.999 9), 13.69 ~ 273.8 mg · L -1 (r = 0.999 5), 2.350 ~ 47.00 mg · L -1 ( r = 0.999 4). The average recoveries were 97.97%, 99.88%, 98.79%, 97.12%, 98.21%, 100.02%, 98.96% and 99.35%, respectively. The RSDs were 1.49%, 0.81% 1.10%, 0.84%, 1.61%, 0.78%, 1.79% and 1.52%. Conclusion The established HPLC wavelength switching combined with gradient elution method for the simultaneous determination of eight components in the liver tablets, the method of high sensitivity, high accuracy, specificity, and provide the basis for the quality control of liver tablets.