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用血吸虫成虫冰冻切片间接免疫酶染法(IIP-AWA)检测血清抗血吸虫成虫抗体(AWAb)。5年前已消灭血吸虫病的上海县COPT阳性血清229份和有治疗史COPT阴性血清135份的阳性率各为96.9％和5.2％;122份粪检阳性患者血清均为强阳性;80份健康人血清均阴性。阳性血清滴度≥1:16时,成虫切片多数呈全虫弥漫型显色。149例血清阳性者经吡喹酮治疗后的阴转率,1年内IIP-AWA为80％,明显高于COPT(60.1％);2.5年两者无差别(85.5％与83.6％)。未转阴者滴度明显下降,染色多呈局限型(肠管显色)。表明血清AWAb阳性者,体内可能仍有成虫残存。本法对清查隐性血吸虫病患者(轻度感染,无临床表现者)有实用价值。
Serum anti-schistosome antibody (AWAb) was detected by indirect immunoenzymatic staining (IIP-AWA) using Schistosoma japonicum adult frozen sections. The positive rates of 229 COPT-positive sera and 135 negative-sera of 135 patients with schistosomiasis-eradicated schistosomiasis 5 years ago were 96.9% and 5.2%, respectively; 122 sera were positive for positive sera and 80 were healthy Human serum were negative. Positive serum titer ≥ 1: 16, adult worm was diffuse majority of the full color. The negative conversion rate of IIP-AWA was significantly higher in COPD patients (P <0.05). The IIP-AWA was higher in COPD patients than in those with COPD (60.1%). Non-negative titers decreased significantly, staining mostly confined (intestinal color). Serum AWAb positive, the body may still have adult worms remain. This Law is of practical value in the inventory of patients with latent schistosomiasis (mild infection, no clinical manifestation).