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目的:探讨血管生成与喉鳞状细胞癌自发性细胞凋亡及临床病理特征的关系。方法:采用免疫组织化学SABC方法及原位组织尿嘧啶脱氧核糖核苷酸末断转移酶介导的缺口末断标记(TUNEL)技术检测34例喉鳞状细胞癌中瘤内微血管密度(IMVD)、细胞凋亡指数(AI)及血管内皮生长因子(VEGF)的表达。结果:34例喉鳞状细胞癌中平均IMVD为21.50±8.87,AI中位数为1.15%。有、无颈淋巴结转移组平均IMVD分别为26.33±9.70、17.68±6.06,其差异有统计学意义(P<0.01);组织学分级高、中、低分化组平均IMVD差异有统计学意义(P<0.01),两两比较,高、中分化组差异有统计学意义(P<0.05);VEGF表达与IMVD呈显著正相关(r=0.51,P<0.01);IMVD与AI呈显著负相关(r=-0.53,P<0.01);IMVD与喉癌T分期无关。结论:IM-VD可作为预测喉癌颈淋巴结转移的重要指标;VEGF是一个重要的血管生成因子,可促进喉癌血管生成;血管生成可抑制喉癌自发性细胞凋亡,从而促进喉癌的恶性进展。
Objective: To investigate the relationship between angiogenesis and spontaneous apoptosis of laryngeal squamous cell carcinoma and its clinicopathological features. Methods: Thirty-four specimens of laryngeal squamous cell carcinoma (IMM) were examined by immunohistochemical SABC method and in situ tissue-specific uracil deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) , Apoptosis index (AI) and vascular endothelial growth factor (VEGF) expression. Results: The average IMVD in 34 cases of laryngeal squamous cell carcinoma was 21.50 ± 8.87 and the median of AI was 1.15%. The mean IMVD of the patients with and without cervical lymph node metastasis was 26.33 ± 9.70 and 17.68 ± 6.06, respectively, with significant difference (P <0.01) (P <0.05). There was a significant positive correlation between VEGF expression and IMVD (r = 0.51, P <0.01). There was a significant negative correlation between IMVD and AI r = -0.53, P <0.01). IMVD was not related to T stage of laryngeal cancer. Conclusion: IM-VD can be used as an important index in predicting the cervical lymph node metastasis of laryngeal carcinoma. VEGF is an important angiogenic factor that can promote laryngeal angiogenesis. Angiogenesis can inhibit spontaneous apoptosis of laryngeal carcinoma, Malignant progress.