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高剂量rHuIL-2可诱导人胸腺细胞分化为具有NK和LAK活性的杀伤细胞。本文对用rHuIL-2诱导人胚胸腺细胞NK和LAK活性与胎龄的关系以及对诱导的杀伤细胞的形态学和表型进行了研究。结果表明:①高剂量rHuIL-2可诱导人胚胸腺细胞分化为具有NK和LAK活性的杀伤细胞。②小于16周龄的胎儿胸腺细胞经过rHuIL-2诱导后,不仅诱导的活化细胞数少,而且NK和LAK功能明显低于24周龄以上的胎儿胸腺细胞;24周以上胎龄的胎儿胸腺细胞经rIL-2诱导后的NK和LAK活性接近新生儿。③rIL-2诱导的人胚胸腺细胞形态学上主要为大颗粒淋巴细胞,其表型为NKH_1~+,CD_1(?)_-的细胞。此结果不仅对于深入了解不同胎龄胸腺绍胞在功能上的个体发育程度提供了客观的指标,而且对于选择适当胎龄胎儿胸腺细胞诱导LAK和NK细胞,作为恶性肿瘤的生物治疗以及探讨外周血NK细胞的来源均有一定的意义。
High-dose rHuIL-2 induces the differentiation of human thymocytes into NK and LAK-activating killer cells. In this paper, the relationship between rHuIL-2 induced NK and LAK activity in human embryonic thymocytes and gestational age and the morphology and phenotype of induced killer cells were studied. The results showed that: ①high dose of rHuIL-2 induced human embryonic thymocytes to differentiate into killer cells with NK and LAK activity. (2) Fetal thymocytes less than 16 weeks of age induced by rHuIL-2, not only induced a small number of activated cells, and NK and LAK function was significantly lower than 24 weeks of age fetal thymocytes; 24 weeks of fetal thymus The NK and LAK activities induced by rIL-2 are close to the newborn. (3) The morphology of human embryonic thymocytes induced by rIL-2 is mainly large granular lymphocytes, whose phenotype is NKH_1 ~ +, CD_1 (?) _-. This result not only provides an objective index for understanding the functional development of thymus cells in different gestational age, but also induces LAK and NK cells to select suitable fetal fetal thymocytes for biological treatment of malignant tumors and to explore the relationship between peripheral blood The origin of NK cells have some significance.