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目的探讨1,25-二羟基维生素D3[1,25(OH)2D3]对原发性骨质疏松骨代谢的影响。方法采用8月龄雌性新西兰兔,摘除双侧卵巢建立Ⅰ型骨质疏松模型,随机分为假手术组(Sham)、单纯去卵巢组(OVX)、葡萄糖酸钙组(OC)和葡萄糖酸钙+1,25(OH)2D3组(OCR)。采用3岁龄新西兰雌兔和4岁龄新西兰雄兔,作为Ⅱ型骨质疏松动物模型,随机分为空白对照组(Con-trol)、葡萄糖酸钙组(Calc ium)、葡萄糖酸钙+1,25(OH)2D3组(CR)。OC组和Calc ium组单纯给予葡萄糖酸钙,OCR组和CR组给予葡萄糖酸钙和1,25(OH)2D3。给药8周后测定各实验组的骨代谢生化指标。结果给药8周后,在Ⅰ型骨质疏松模型中,OCR组的血钙、血磷、碱性磷酸酶(ALP)均较OVX组和OC组显著升高(P<0.05或P<0.01),OCR组的BGP、尿Ca/Cr较OVX组和OC组明显降低(P<0.05或P<0.01)。在Ⅱ型骨质疏松模型中,雌、雄性兔CR组的血钙、BGP、ALP均较Control组和Calc ium组有显著性升高(P<0.05或P<0.01),尿Ca/Cr较其他两组明显下降(P<0.05或P<0.01)。结论1,25(OH)2D3对Ⅰ型骨质疏松症有降低骨转换,促进骨形成,抑制骨吸收的作用;对Ⅱ型骨质疏松症有提高骨转换,促进骨形成,抑制骨吸收的作用。
Objective To investigate the effects of 1,25-dihydroxyvitamin D3 (1,25 (OH) 2D3] on bone metabolism in primary osteoporosis. Methods Female New Zealand rabbits aged 8 months were divided into ovariectomized group (OVX), calcium gluconate (OC) and calcium gluconate +1,25 (OH) 2D3 group (OCR). Three-year-old New Zealand female rabbits and 4-year-old New Zealand male rabbits were randomly divided into control group (Con-trol), calcium group (Calcium), calcium gluconate , 25 (OH) 2D3 group (CR). Calcium gluconate was given to the OC and Calcium groups, while calcium gluconate and 1,25 (OH) 2D3 were given to the OCR and CR groups. After 8 weeks of administration, the biochemical indexes of bone metabolism in each experimental group were determined. Results After 8 weeks of administration, the levels of serum calcium, phosphorus, and alkaline phosphatase (ALP) in OCR group were significantly higher than those in OVX and OC groups in type Ⅰ osteoporosis model (P <0.05 or P <0.01 ). BGP and urinary Ca / Cr in OCR group were significantly lower than those in OVX and OC groups (P <0.05 or P <0.01). In type Ⅱ osteoporosis model, serum calcium, BGP and ALP in CR group were significantly higher than those in Control group and Calcium group (P <0.05 or P <0.01) The other two groups decreased significantly (P <0.05 or P <0.01). Conclusions 1,25 (OH) 2D3 has the effect of reducing bone turnover, promoting bone formation and inhibiting bone resorption in type Ⅰ osteoporosis. For type Ⅱ osteoporosis, there is an increase in bone turnover, bone formation and bone resorption effect.