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目的:探讨热休克蛋白27(HSP27)在鼠青光眼模型视网膜神经节细胞(RGCs)中的表达以及眼压对抗HSP27自身抗体的影响。方法:使用SPSS12.0软件将55只Wistar大鼠随机分为高眼压组(25只鼠)、sham对照(假手术)组(25只鼠)及正常对照组(5只鼠)。采用电凝鼠巩膜表面至少3组静脉及角膜缘周围血管,建立鼠青光眼模型。采用免疫组化和酶联免疫吸附测定(ELISA)方法分别检测术后1,2,3,4及8wk视网膜中RGCs以及神经纤维层(RNFL)HSP27的表达、分布以及血清中抗HSP27抗体水平。结果:随着眼压升高及高眼压持续时间延长,高眼压组右眼RGCs中HSP27表达逐渐增强,与其左眼、sham对照组右、左眼和正常对照组右、左眼比较,差异均有统计学意义(P<0.001),且RNFL中也出现HSP27的表达。高眼压组血清中抗HSP27抗体水平在术后1wk轻度升高(P>0.05),随着眼压升高及高眼压持续至术后8wk,血清中HSP27抗体水平逐渐升高并稳定于较高水平,与sham对照组和正常对照组比较,差异有统计学意义(P<0.05)。结论:内源性HSP27表达增强可能在青光眼视神经病变中具有重要作用。
Objective: To investigate the expression of heat shock protein 27 (HSP27) in rat retinal ganglion cells (RGCs) and the effect of intraocular pressure (IOP) on autoantibodies against HSP27. Methods: 55 Wistar rats were randomly divided into high intraocular pressure (25 rats), sham control (sham) group (25 rats) and normal control (5 rats) using SPSS 12.0 software. At least three groups of veins and peri-limbal blood vessels were electrochemically scleroded to establish a rat glaucoma model. Immunohistochemistry and enzyme-linked immunosorbent assay (ELISA) were used to detect the expression and distribution of RG27 and RNFL HSP27 in retina at 1, 2, 3, 4 and 8w after operation respectively. Results: With the increase of intraocular pressure (IOP) and duration of elevated intraocular pressure (IOP), the expression of HSP27 in right ocular RGCs increased gradually compared with the right and left eyes in the left eye and the sham control group Were statistically significant (P <0.001), and RNFL also appeared in the expression of HSP27. The level of anti-HSP27 antibody in the serum of patients with ocular hypertension increased slightly at 1w after operation (P> 0.05). With the rise of intraocular pressure and ocular hypertension until 8wk after operation, the level of HSP27 antibody in serum increased gradually and stabilized at Higher level, compared with sham control group and normal control group, the difference was statistically significant (P <0.05). Conclusion: The enhanced expression of endogenous HSP27 may play an important role in glaucomatous optic neuropathy.