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目的观察三七总皂苷对3T3-L1细胞增殖、分化和分泌Leptin、PAI-1的影响,探讨其调脂作用的可能机制。方法培养3T3-L1细胞,并用不同浓度的三七总皂苷(5,50,100μg.mL-1)进行干预,以四甲基偶氮唑盐(MTT)法检测细胞增殖;用油红O染色和染色比色法分析脂肪细胞的分化程度,用逆转录多聚酶联反应(RT-PCR)检测脂肪细胞分化相关基因过氧化物酶增殖物激活受体γ(2PPARγ)2、CCAAT/增强子结合蛋白α(C/EBPα)mRNA的表达;用ELISA法检测细胞培养上清中Leptin、PAI-1的含量。结果三七总皂苷低、中浓度组对模型细胞增殖无明显影响(P>0.05),高浓度组能促进细胞的增殖(P<0.05);中、高浓度组明显抑制模型细胞的分化,并能抑制PPARγ2、C/EBPα基因的表达(P<0.01);药物对模型细胞分泌Leptin、PAI-1有明显抑制作用(P<0.01)。结论三七总皂苷可能通过抑制3T3-L1前脂肪细胞的分化和抑制其分泌因子而达到调脂作用,以中剂量作用效果最好。
Objective To observe the effect of Panax notoginseng saponins on the proliferation, differentiation and secretion of Leptin and PAI-1 in 3T3-L1 cells and to explore the possible mechanism of its regulation. Methods 3T3-L1 cells were cultured and treated with different concentrations of Panax notoginseng saponins (5, 50 and 100 μg.mL-1). Cell proliferation was detected by MTT assay. The differentiation degree of adipocytes was analyzed by staining colorimetric assay. The expressions of adipocyte differentiation-related gene peroxisome proliferator-activated receptor γ (2PPARγ) 2, CCAAT / enhancer binding protein α (C / EBPα) mRNA expression. The contents of Leptin and PAI-1 in cell culture supernatants were detected by ELISA. Results Panax notoginseng saponins had no significant effect on the proliferation of model cells (P> 0.05), while the high concentration group could promote the proliferation of cells (P <0.05). The medium and high concentration groups significantly inhibited the differentiation of model cells Can inhibit the expression of PPARγ2 and C / EBPα gene (P <0.01). The drug significantly inhibited the secretion of Leptin and PAI-1 in model cells (P <0.01). Conclusion Panax notoginseng saponins may achieve lipid-lowering effect by inhibiting the differentiation of 3T3-L1 preadipocytes and inhibiting its secretion factors, and the best effect is achieved by medium dose.