论文部分内容阅读
目的为了解广州地区庚型肝炎病毒(HGV)感染的现状和流行特点,我们对348例住院和门诊的肝病患者进行了HGV RNA、HGV抗体(HGV-Ab)检测,现将结果报道如下。材料和方法从广东省人民医院门诊和住院肝病患者收集血清标本348份,其中丙型肝炎93份,乙型肝炎120份,肝硬化75份,肝细胞癌60份。采用逆转录套式PCR检测HGV RNA。引物序列为:HGV1(F外)5’-CGCTCAAGCCAGCGAAGTAAGCA-3’,HGV_2(R外)5’-CAATACCTCTCACCGACGGG-3’,HGV3(F内)5’-GGACTTCCGGATAGCTGAAAGCT-3’,HGV4(R内)5’-GCGTCCACACAGATGGCGA-3’。两次 PCR循环条件相同,均为:94℃ 1min、55℃1min、72℃ 1min,35个循环。产物进行琼脂糖凝胶电泳,紫外灯下观察结果。采用酶联免疫吸附试验(ELISA)检测HGV-Ab。诊断试剂为北京大学医学院产品。结果 348份标本中,HGV RNA阳性检出率为8.62％(30/348)。其中丙肝标本检出率为6.45％(6/93),乙肝标本检出率为7.50％(9/120),肝硬化标本检出率为8.00％(6/75),肝细胞癌标本检出率为15.00％(9/60)。HGV-Ab阳性检出率为12.93％(45/348)。其中丙肝标本检出率为12.90％(12/93),乙肝标本检出率为10.00％(12/120),肝硬化标本检出率为8.00％(6/75),肝细胞癌标本检出率为25.00％(15/60)。30份HGVRNA阳性标本中HGV-Ab均为阳性。结论 HGV在广州地区多种肝病人群中的感染状况较普遍,与其它肝病重叠感染的情况较严重。应得到足够重视。
Objective To understand the prevalence and epidemic characteristics of hepatitis G virus (GGV) infection in Guangzhou, HGV RNA and HGV antibody (HGV-Ab) were detected in 348 inpatients and outpatients with liver disease. The results are reported as follows. MATERIALS AND METHODS: A total of 348 serum samples were collected from outpatients and hospitalized patients with liver disease in Guangdong Provincial People’s Hospital. Among them, 93 were hepatitis C, 120 were hepatitis B, 75 were cirrhosis and 60 were hepatocellular carcinoma. HGV RNA was detected by reverse transcription PCR. The primer sequences were: 5’-CGCTCAAGCCAGCGAAGTAAGCA-3 ’for HGV1 (ex F), 5’-CAATACCTCTCACCGACGGG-3’ for HGV_2 (exo), 5’GGACTTCCGGATAGCTGAAAGCT-3 ’for HGV3 -GCGTCCACACAGATGGCGA-3 ’. The two PCR cycles were the same with the following conditions: 94 ° C for 1 min, 55 ° C for 1 min, and 72 ° C for 1 min for 35 cycles. The product was subjected to agarose gel electrophoresis and the result was observed under a UV lamp. HGV-Ab was detected by enzyme-linked immunosorbent assay (ELISA). Diagnostic reagents for Peking University School of Medicine products. Results Among 348 samples, the positive rate of HGV RNA was 8.62% (30/348). Among them, the detection rate of hepatitis C was 6.45% (6/93), the detection rate of hepatitis B was 7.50% (9/120), the detection rate of cirrhosis was 8.00% (6/75) The rate was 15.00% (9/60). The positive rate of HGV-Ab was 12.93% (45/348). Among them, the detection rate of hepatitis C was 12.90% (12/93), the detection rate of hepatitis B was 10.00% (12/120), the detection rate of cirrhosis was 8.00% (6/75) The rate was 25.00% (15/60). HGV-Ab was positive in 30 HGV RNA positive samples. Conclusion The prevalence of HGV in various liver diseases in Guangzhou is more common than other liver diseases. Should receive enough attention.