论文部分内容阅读
目的:研究喉鳞状细胞癌(LSCC)中血管生成素-1,2(Ang-1,2)的表达及与临床病理学特征、CD105标记的微血管密度(MVD)之间的关系,评估Ang-1,2及血管内皮生长因子(VEGF)在LSCC中的联合表达对肿瘤血管生成的作用。方法:用常规免疫组织化学法检测LSCC标本61例、癌旁组织30例及正常喉黏膜组织15例中Ang-1,2及VEGF的表达和MVD,并对临床病理资料进行回顾性分析。结果:Ang-1,2在LSCC细胞、正常喉黏膜上皮细胞及血管内皮细胞均有表达;VEGF只表达于LSCC组织中;LSCC组织与癌旁组织及正常喉黏膜组织相比,Ang-1,2表达显著增加(均P<0.05)。对于临床病理因素的分析表明,Ang-1,2在高、中低分化LSCC中的表达差异无统计学意义(均P<0.05);Ang-2表达与临床分期、MVD有显著的相关性(均P<0.05),而Ang-1的表达与临床分期、MVD无显著的相关性(均P>0.05)。在所有联合表达状态中Ang-2和VEGF同时为阳性的肿瘤组织MVD均值为最高,显著高于其他任何状态(P<0.05)。结论:Ang-1,2在LSCC中的表达可能在肿瘤进展过程中起了重要的作用,与肿瘤血管的形成关系密切。其中Ang-2与VEGF共同作用促进肿瘤血管的形成。
Objective: To investigate the relationship between the expression of Ang-1,2 in laryngeal squamous cell carcinoma (LSCC) and clinicopathological features and the expression of CD105-labeled microvessel density (MVD) 1, 2 and vascular endothelial growth factor (VEGF) in LSCC on tumor angiogenesis. Methods: The expressions of Ang-1, 2 and VEGF and MVD in 61 cases of LSCC, 30 cases of adjacent tissues and 15 cases of normal laryngeal mucosa were detected by routine immunohistochemistry. The clinical and pathological data were retrospectively analyzed. Results: Ang-1 was expressed in LSCC cells, normal laryngeal mucosa epithelial cells and vascular endothelial cells, while VEGF was only expressed in LSCC tissues. Compared with adjacent normal tissues and normal laryngeal mucosa tissues, Ang-1, 2 expression increased significantly (both P <0.05). The analysis of clinicopathological factors showed that there was no significant difference in the expression of Ang-1,2 in high and moderately differentiated LSCC (all P <0.05), and there was a significant correlation between Ang-2 expression and clinical stage and MVD P <0.05). However, there was no significant correlation between Ang-1 expression and clinical stage and MVD (all P> 0.05). The mean MVD of tumor tissues with both Ang-2 and VEGF positive in all co-expression was the highest, significantly higher than any other state (P <0.05). CONCLUSION: The expression of Ang-1,2 in LSCC may play an important role in tumor progression and is closely related to the formation of tumor blood vessels. Among them, Ang-2 cooperates with VEGF to promote the formation of tumor blood vessels.