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目的:研究过表达小鼠树突状细胞表面受体Fpr2对树突状细胞成熟和趋化功能的影响.方法:通过RVG-P靶向多肽介导质粒PEGFP-N1-Fpr2转染小鼠树突状细胞,上调表面受体Fpr2的表达.ELISA检测IL-6、IL-10,IL-12 p70及TNF-α的水平;通过流式细胞术检测细胞表面分子CD40,CD80,CD83,CD86,MHCII的表达;使用Transwell小室检测细胞迁移能力;Western blot检测MAPK通路(P38,ERK1/2,JNK)的磷酸化情况.结果:上调Fpr2后,表面分子CD83的表达水平明显升高.在细胞因子水平上,转染PEGFP-N1-Fpr2组的树突状细胞的IL-6,IL-10,TNF-α分泌量明显增多.转染质粒DNA后,细胞对CCL21的趋化减弱,但与转染空载体pEGFP-N1组相比,转染pEGFP-N1-Fpr2组的趋化细胞数明显增多.转染pEGFP-N1-Fpr2组的P38,JNK及ERK1/2磷酸化水平均显著高于转染pEGFP-N1组.结论:过表达细胞表面受体Fpr2能促进树突状细胞的成熟,并可能通过上调MAPK信号通路中的P38和JNK的磷酸化来增加其趋化能力.“,”Objective:To investigate the regulatory effect of over-expression of Formyl Peptide Receptor-2(Fpr2)on dendritc cell maturation and chemotaxis. Methods:Dendritic cell line DC2. 4 was transfected with plasmid DNA pEGFP-Fpr2 to upregulate the expression of Fpr2 with the aid of a targeted peptide RVG-P. The concentration of selected cytokines(IL-6, IL-10,TNF-α,IL-12 p70)was measured by using the ELISA KIT. The expression levels of surface marker molecules(CD40, CD80,CD83,CD86,MHCII) were measured by FACS. Migration assay was performed by using Transwell migration cham-bers. Western blot was performed to evaluate the phosphorylated forms of intracellular signal transducers of MAPK ( P38, ERK1/2,JNK). Results:The expression levels of CD83 in DC2. 4 increased upon up-regulation of Fpr2. The cytokine levels of IL-6,IL-10 and TNF-α increased after up-regulation of Fpr2. The average number of cell migration decreased after plas-mid DNA transfection. However,the migration ability of pEGFP-Fpr2 transfected cells increased compared with the pEGFP-N1 transfected cells. Phosphorylation levels of P38,JNK and ERK1/2 in the pEGFP-Fpr2 transfected cells were observed with a significant increase when compared with the pEGFP-N1 transfected cells. Conclusion:Over-expression of Formyl Pep-tide Receptor-2 promoted maturation and chemotaxis of dendritic cells.