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目的:初步探讨不同剂量三聚氰胺对SD雌鼠卵巢的毒性作用。方法:将60只SD雌性大鼠随机分A、B、C、D四组,A、B、C为实验组,D为对照组。分别予以400 mg/kg、800 mg/kg、1 600 mg/kg的三聚氰胺灌胃,1次/日,持续35天,用药结束后24 h处死大鼠。切取卵巢称量湿重、做病理切片、记数各级卵泡。取大鼠卵巢组织固定切片,做HE染色;用免疫组化方法检测P450arom在大鼠卵巢中的分布及表达。结果:A、B、C 3组大鼠卵巢湿重与D组相比明显减轻(P<0.05);B、C组卵巢湿重相比无统计学差异,但与A组相比亦有减轻(P<0.05);A、B、C 3组的总卵泡数、成熟卵泡数、初级卵泡数和生长卵泡数明显少于D组(P<0.05)。与对照组比较,各实验组P450表达强度减弱(P<0.05);B组和C组各级卵泡数均减少,B组伴间质及颗粒细胞的崩解坏死;A组生长卵泡、成熟卵泡数均明显减少。结论:三聚氰胺所致的卵巢毒性与摄入量有一定的相关性。过量的三聚氰胺能够抑制鼠体内细胞色素芳香化酶P450的表达,使体内E2生成障碍,从而影响卵泡发育。
Objective: To investigate the toxic effects of different doses of melamine on the ovaries of female SD rats. Methods: Sixty female SD rats were randomly divided into four groups: A, B, C, D, A, B and C as experimental group and D as control group. Rats were administered with 400 mg / kg, 800 mg / kg, and 1 600 mg / kg of melamine once daily for 35 days. Rats were sacrificed 24 h after the end of treatment. Cut the ovary weighing wet weight, make pathological sections, count follicles at all levels. The ovariectomized sections of rat ovaries were taken for HE staining. The distribution and expression of P450arom in ovary of rats were detected by immunohistochemistry. Results: The ovarian wet weight of rats in groups A, B and C was significantly reduced compared with that of group D (P <0.05), but there was no significant difference in wet weight of ovaries between groups B and C (P <0.05). The total number of follicles, mature follicles, primary follicles and growing follicles in group A, B and C 3 were significantly less than those in group D (P <0.05). Compared with the control group, the expression of P450 in each experimental group was weakened (P <0.05); the number of follicles at all levels in group B and group C were decreased; the group B was disintegrated and necrotic with interstitial and granulosa cells; the group A grew follicles and mature follicles The number were significantly reduced. Conclusion: The ovarian toxicity induced by melamine is related to intake. Excess melamine can inhibit the expression of cytochrome aromatase P450 in mice and cause the formation of E2 in the body, thereby affecting follicular development.