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为了观察慢性脑缺血时海马CA1区中BDNF的表达变化,探讨缺血性脑损伤及修复机制。我们将Wistar大鼠双侧颈总动脉结扎制成慢性脑缺血动物模型。分为三组:正常对照组、慢性脑缺血30d和120d组。分别于术后30d和120d处死动物,行BDNF免疫组化染色,计数各组大鼠海马CA1区中的阳性神经元数。结果显示:(1)在对照组的海马CA1区可见BDNF较强的表达;(2)在慢性脑缺血30d时,海马CA1区BDNF的表达高于缺血120d组及对照组(P<0.05)。而120d时,海马CA1区BDNF的表达与对照组无显著性差异(P>0.05)。结果提示:(1)在正常大鼠海马CA1区有BDNF表达,能维持神经元的存活;(2)慢性脑缺血时,BDNF在海马CA1区的表达先升后降。
In order to observe the changes of BDNF expression in hippocampal CA1 region during chronic cerebral ischemia, the mechanism of ischemic brain injury and its repair were explored. We will Wistar rat bilateral common carotid artery ligation made of chronic cerebral ischemia model. Divided into three groups: normal control group, chronic cerebral ischemia 30d and 120d group. Animals were sacrificed at 30 days and 120 days after operation, respectively. BDNF immunohistochemical staining was used to count the number of positive neurons in CA1 area of hippocampus. The results showed that: (1) BDNF was found in hippocampal CA1 region of the control group; (2) BDNF expression in CA1 hippocampus was higher than that in the 120th and the control group (P <0.05 ). At 120 days, the expression of BDNF in hippocampal CA1 region was not significantly different from that in control group (P> 0.05). The results suggest that: (1) BDNF expression in normal rat hippocampal CA1 area, can maintain the survival of neurons; (2) chronic cerebral ischemia, BDNF in hippocampal CA1 area first and then decreased.