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目的:研究原发性肝癌组织中树突状细胞(DC)浸润与肝癌临床病理特征、生物学行为、预后之间的关系,明确DC浸润对肝癌发生发展及预后的影响。方法:用免疫组化(即用型两步法SuperveisionTM)法检测60例原发性肝癌术后组织标本中DC标志蛋白S-100和增殖细胞核抗原(PCNA)的表达;以原位末端标记技术(TUNEL)检测肝癌细胞凋亡比例。结果:高分化肝癌组织中DC浸润数量高于低分化肝癌(P<0.05);在无门静脉侵犯或淋巴结转移的肝癌组织中DC浸润数量显著高于有门静脉侵犯或淋巴结转移的肝癌组织(P<0.05);DC高浸润组的晚期肝癌比例低于DC低浸润组(P<0.05);DC高浸润组预后好于DC低浸润组(P<0.05)。DC浸润与增殖细胞核抗原标记指数(PCNA-LI)呈负线性相关(r=-0.337,P<0.01),与凋亡细胞指数(AI)呈正线性相关(r=0.435,P<0.01)。结论:DC浸润与肝癌的生物学行为密切相关,DC在肝癌的发生发展和预后中有重要作用,抑制肝癌细胞增殖、促进肝癌细胞凋亡是其可能的作用机制之一。
Objective: To study the relationship between dendritic cell (DC) infiltration in primary hepatocellular carcinoma and the clinicopathological features, biological behavior and prognosis of hepatocellular carcinoma and to clarify the effect of DC infiltration on the occurrence, development and prognosis of hepatocellular carcinoma. Methods: The expression of DC marker protein S-100 and proliferating cell nuclear antigen (PCNA) in 60 cases of primary hepatocellular carcinoma tissues were detected by immunohistochemistry (ie, using the two-step SuperveisionTM) method. (TUNEL) detection of hepatocellular carcinoma apoptosis ratio. Results: The number of DC infiltration in well-differentiated HCC was higher than that in poorly-differentiated HCC (P <0.05). The number of DC infiltration in HCC without portal vein invasion or lymph node metastasis was significantly higher than that in HCC with portal vein invasion or lymph node metastasis (P < 0.05). The proportion of advanced hepatocellular carcinoma in DC group was lower than that in DC group (P <0.05). The prognosis of DC group was higher than that of DC group (P <0.05). DC infiltration was negatively correlated with proliferating cell nuclear antigen index (PCNA-LI) (r = -0.337, P <0.01), and positively correlated with apoptotic cell index (AI) (r = 0.435, P <0.01). Conclusion: The infiltration of DC is closely related to the biological behavior of hepatocellular carcinoma. DC plays an important role in the development and prognosis of hepatocellular carcinoma. It is one of the possible mechanisms that DC can inhibit the proliferation of hepatoma cells and promote the apoptosis of hepatoma cells.