细胞DNA倍体分析法[1]是一项用于肿瘤早期诊断的新技术,该技术将Feulgen染色法与计算机图像分析技术相结合。Feulgen染色对显示细胞核DNA含量既精确又具有一定的特异性[2],其原理是利用硫堇等染料对细胞核DNA产生特异性着色;细胞核着色的深浅代表DNA含量的多少,将着色强弱不等的细胞核通过自动扫描显微镜转化为光密度值(IOD),通过计算机及显微图像分析技术进行整合、处理。细胞DNA倍体分析技术已应用于妇科及非妇科临床细胞学检测,许多研究显示[1,3,4]在宫颈癌及癌前病变筛查中有较高的敏感性。但此项技术质控难度大,检测结果易受标本取材、制作干片质量、显微镜扫描及数据分析等诸多因素 Cell DNA ploidy assay [1] is a new technique for the early diagnosis of tumors, which combines Feulgen staining with computer image analysis techniques. Feulgen staining shows that the DNA content of the nucleus is both accurate and specific [2]. The principle is that the nucleus DNA is specifically colored with a dye such as thionine. The depth of the nucleus coloring represents the amount of DNA content, Such as the nucleus by automatic scanning microscope into optical density (IOD), through computer and microscopic image analysis technology integration and processing. Cell DNA ploidy analysis technology has been used in gynecological and gynecological clinical cytology, many studies have shown [1,3,4] in cervical cancer and precancerous lesions screening has a higher sensitivity. However, this technology is difficult to control the quality of the test results are susceptible to specimen preparation, the quality of dry film production, microscopy and data analysis and many other factors