论文部分内容阅读
目的观察褪黑素(MLT)对糖尿病大鼠早期视网膜神经组织神经胶质原纤维酸性蛋白(GFAP)表达的影响。方法 SD大鼠尾静脉注射适量2%STZ溶液制造糖尿病模型。随机分成3组:正常对照组、模型对照组和MLT治疗组,每组12只。MLT治疗组的大鼠给予腹腔注射MLT10mg/(kg·d);正常对照组和模型对照组大鼠每天腹腔注射等量的0.9%氯化钠溶液。每组于4、6、8周各取4只大鼠分别应用Western Blotting和免疫组织化学方法定量定性检测视网膜神经组织GFAP的表达。结果模型对照组于4、6、8周时的视网膜神经组织GFAP阳性表达量分别是0.0887±0.0239、0.1057±0.0282和0.2039±0.0228,均明显高于同时相的正常对照组(分别是0.0452±0.0089、0.0479±0.0100和0.0526±0.0165)和MLT治疗组(分别是0.0485±0.0149、0.0510±0.0144和0.0722±0.0228),差异有统计学意义(P<0.01);而MLT治疗组视网膜神经组织GFAP阳性表达与正常对照组相比,4、6周时差异无统计学意义,8周时高于同时相的正常对照组(P<0.05)。结论 MLT可降低糖尿病大鼠视网膜神经组织GFAP的阳性表达,减轻视网膜神经组织的退行性变。
Objective To observe the effect of melatonin (MLT) on the expression of glial fibrillary acidic protein (GFAP) in the early retina of diabetic rats. Methods SD rats were injected into tail vein with 2% STZ solution to make diabetic model. Randomly divided into 3 groups: normal control group, model control group and MLT treatment group, 12 rats in each group. The rats in MLT group were intraperitoneally injected with MLT 10 mg / (kg · d). The normal control group and model control group were injected with 0.9% sodium chloride solution daily. At 4, 6 and 8 weeks, 4 rats in each group were used to quantitatively detect the expression of GFAP in retinal nerve tissue by Western Blotting and immunohistochemistry respectively. Results The GFAP positive expression of retinal nerve tissue in the model control group at 4, 6 and 8 weeks were 0.0887 ± 0.0239, 0.1057 ± 0.0282 and 0.2039 ± 0.0228, respectively, which were significantly higher than that of the normal control group (0.0452 ± 0.0089 , 0.0479 ± 0.0100 and 0.0526 ± 0.0165 respectively) and MLT treatment group (0.0485 ± 0.0149,0.0510 ± 0.0144 and 0.0722 ± 0.0228 respectively), the difference was statistically significant (P <0.01), while MLT treatment group showed positive expression of GFAP in retinal nerve tissue Compared with the normal control group, there was no significant difference at 4 and 6 weeks, and higher than the normal control group at 8 weeks (P <0.05). Conclusion MLT can reduce the positive expression of GFAP in the retina of diabetic rats and reduce the degeneration of the retina.