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目的观测氯硝柳胺展膜剂水面杀日本血吸虫尾蚴的效果。方法 1)直接杀蚴试验。取3047FALCON细胞培养盒,注入去氯水,放入尾蚴,再滴加3.4、1.7、0.8、0.4和0.2μl,氯硝柳胺展膜剂,分别记录5、10和30 min尾蚴死亡数。2)室内杀蚴感染实验。取4只圆形塑料盆,注入去氯水15 L,水面积为0.163 m2。每盆移入尾蚴1 500条,静置0.5h。试验组3盆,氯硝柳胺展膜剂浓度分别为0.2、0.3和0.4 g/m2,施药5 min后,各放入小鼠20只感染30 min,同时设清水对照组,感染完成后放动物室进行常规饲养,35 d后剖杀,收集成虫计数。3)现场模拟试验。选择长为50 m,宽为2 m的沟渠2条,分别放置40只阳性钉螺逸蚴30 min后,试验组喷洒1%氯硝柳胺展膜剂0.3 g/m2,两组各放置小鼠20只/笼,每天感染4 h,连续2 d,室内饲养35 d后解剖。结果直接杀蚴试验1%氯硝柳胺展膜剂用量为3.4、1.7、0.8、0.4和0.2μl时,5 min尾蚴死亡率分别为97.83%、100%、26.67%、11.86%和18.84%;10 min分别为100%、100%、93.33%、74.19%和43.84%;30 min均为100%。室内杀蚴感染实验1%氯硝柳胺展膜剂用量为0.2、0.3、0.4 g/m2浓度时,小鼠感染率均为0%,清水对照组小鼠感染率为100%。模拟现场试验试验组小鼠感染率均为0%,对照组感染率47.37%。结论1%氯硝柳胺展膜剂现场喷洒杀尾蚴用量小,在水面铺展速度快,0.2 g/m2以上浓度杀蚴率100%。
Objective To observe the effect of niclosamide enveloping agent on killing Cercaria. Method 1) direct killing of larvae test. Take 3047FALCON cell culture box, into the dechlorinated water, into the cercariae, followed by dropwise addition of 3.4,1.7,0.8,0.4 and 0.2μl of niclosamide film-forming agent were recorded 5,10 and 30 min cercariae deaths. 2) Indoor kill larvae infection experiment. Take 4 round plastic basin, into the chlorine water 15 L, the water area of 0.163 m2. Each pot into the cercariae 1 500, standing 0.5h. In the experimental group, the concentration of niclosamide enamelling agent was 0.2, 0.3 and 0.4 g / m2 respectively. Twenty mice in each group were infected for 30 min after 5 min of application. At the same time, a control group was established. After the infection was completed Put animal room for routine feeding, 35 days after the killing, collecting adult count. 3) field simulation test. Two ditches of 50 m in length and 2 m in width were selected and 40 larvae were placed for 30 min. The test group was sprayed with 0.3 g / m2 of niclosamide enveloping agent 0.3 g / m2, 20 / cage, infected 4 h daily for 2 days, 35 days after indoor feeding anatomy. Results The test results showed that the mortality of 5 - month - old cercariae was 1%, 100%, 26.67%, 11.86% and 18.84% when the dosage of 1% niclosamide coating agent was 3.4, 1.7, 0.8, 0.4 and 0.2μl respectively. 10 min were 100%, 100%, 93.33%, 74.19% and 43.84%, respectively; all were 100% in 30 min. Laboratory killing of larvae 1% infection of niclosamide enamelling agent 0.2,0.3,0.4 g / m2 concentration, the mouse infection rate was 0%, the control group of mice infected with water was 100%. The mice in the simulated field test were all infected with 0% infection rate and control group infection rate was 47.37%. Conclusion 1% niclosamide coating agent on-site spraying cercariae dosage is small, spreading speed in the water fast, 0.2 g / m2 concentration above 100% killing rate of larvae.