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目的探讨低剂量环磷酰胺对烧伤大鼠全身炎症介质综合征 (SIRS)的控制作用。方法以大鼠为实验动物 ,分为空白组、对照组、实验组。对照组、实验组制作 5 0 %Ⅲ°烫伤模型 ,实验组于伤后 1小时腹腔内注射低剂量环磷酰胺 (2mg/kg) ,对照组腹腔内注射等量生理盐水。分别在伤后 2h、3h、6h测量 2组血液及肝脏组织中肿瘤坏死因子 (TNF -a)的含量。喂养至动物死亡观察两组动物的生存时间。结果实验组、对照组伤后 2小时血液及肝脏内TNF -a的含量明显高于未烧伤的空白组 (P <0 0 5 )。实验组伤后 2h、3h、6h血液及肝脏内TNF -a的含量明显低于对照组 (P <0 0 5 )。实验组动物的生存时间明显延长 (P <0 0 5 )。结论低剂量环磷酰胺能够减少烧伤后炎症介质的释放 ,能减轻烧伤后SIRS的发生。
Objective To investigate the effect of low dose cyclophosphamide on systemic inflammatory mediator syndrome (SIRS) in burn rats. Methods Rats were used as experimental animals and divided into blank group, control group and experimental group. In the control group and experimental group, a 50% Ⅲ scald model was made. In the experimental group, intraperitoneal injection of low dose cyclophosphamide (2mg / kg) was given at 1 hour after injury and the control group was injected intraperitoneally with the same amount of saline. The levels of tumor necrosis factor (TNF-a) in blood and liver tissues were measured at 2h, 3h, 6h after injury respectively. Feeding to animal death observed the survival time of two groups of animals. Results The contents of TNF-a in blood and liver in experimental group and control group were significantly higher than those in non-burned blank group at 2 hours after injury (P <0.05). The contents of TNF-a in blood and liver in experimental group at 2h, 3h, 6h after injury were significantly lower than those in control group (P <0.05). The survival time of experimental animals was significantly longer (P <0.05). Conclusion Low-dose cyclophosphamide can reduce the release of inflammatory mediators after burn, and can reduce the incidence of SIRS after burn.