论文部分内容阅读
拟豪猪刺(Berberis soulieana schneid)系小檗科小檗属植物,分布于甘、陕、川、鄂等省。民间有用根及茎皮作黄连代用品,具有抗菌消炎作用。《中药志》仅用薄层色谱定性分析检查出小檗碱,巴马亭和小檗胺。本文从其根的酸水提取部分用硅胶柱色谱分离得B_3,B_4和B_5三个季铵生物碱。小檗碱:R_1R_2=—CH_2—巴马亭:R_1=R_2=—CH_3 药根碱:R_1=—H,R_2=—CH_3 三个化合物具有相似的紫外吸收,λ_(max)~(MeOH) 224,263,247和430nm、λ_(min)~(MeOH) 246,300和377nm,为2、3、9、10—四氧基取代的原小檗碱型骨架特
The Berberis soulieana schneid is a genus of plants belonging to the family Asteraceae, which is distributed in Gansu, Shaanxi, Sichuan and Hubei provinces. Folk roots and stems are used as alternatives to Huanglian, with antibacterial and anti-inflammatory effects. “Chinese Herbal Medicine” only used qualitative analysis of thin-layer chromatography to check berberine, palmatine and berbamine. In this paper, the acid-water extraction fraction of its root was separated by silica gel column chromatography to obtain three quaternary ammonium alkaloids B_3, B_4 and B_5. Berberine: R_1R_2=-CH_2-Parmaine: R_1=R_2=—CH 3 Pharmacoamine: R 1 =—H, R 2 =—CH 3 The three compounds have similar UV absorbance, λ max (MeOH) 224,263,247 And 430nm, λ_(min)~(MeOH) 246, 300, and 377nm, are 2, 3, 9, 10-tetramethoxy substituted protoberberine backbones