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目的建立一种测定血清中1,5-脱水葡萄糖醇(1,5-AG)的方法,辅助诊断糖尿病。方法糖尿病患者(糖尿病组)与健康体检人员(健康对照组)各100例,采用葡萄糖激酶去除血清样品内源葡萄糖干扰,过氧化氢(H_2O_2)在4-氨基替比林(4-AAP)、过氧化物酶(POD)的存在下,可生成红色醌亚胺化合物,发生显色反应进行比色测定。结果该方法最低检测限为1.98μmol/L,1,5-脱水葡萄糖醇在663μmol/L以内线性良好。测定100例糖尿病组患者血清1,5-脱水葡萄糖醇含量为(30.7±16.4)μmol/L,显著低于健康对照组的(170.6±48.2)μmol/L,差异有统计学意义(P<0.05)。结论该法简便快速、灵敏、准确和稳定、特异,可用于糖尿病的及时诊断与监控,适合于各类实验室常规应用。
Objective To establish a method for the determination of 1,5-anhydroglucitol (1,5-AG) in serum to aid in the diagnosis of diabetes. Methods 100 patients with diabetes mellitus (diabetic group) and healthy physical examination group (healthy control group) were treated with glucokinase to remove endogenous glucose, and the effect of hydrogen peroxide (H2O2) on 4-ATI, Peroxidase (POD) in the presence of red quinone imine compounds can be generated, the color reaction occurred colorimetric determination. Results The detection limit of this method was 1.98μmol / L, and the linearity of 1,5-anhydroglucitol was within 663μmol / L. The serum level of 1,5-anhydroglucitol in the 100 diabetic patients was (30.7 ± 16.4) μmol / L, which was significantly lower than that in the healthy controls (170.6 ± 48.2 μmol / L, P <0.05 ). Conclusion The method is simple, rapid, sensitive, accurate, stable and specific. It can be used for timely diagnosis and monitoring of diabetes and is suitable for routine laboratory applications.