论文部分内容阅读
研究了绞股蓝中皂苷的提取分离和纯化工艺。通过绞股蓝水提醇沉,料水比1:10,浓缩后70%醇沉,得多糖蛋白。上清液浓缩回收,分别上D101,LSA-21大孔吸附树脂吸附,经水洗至无色,用30%,50%,,70%,90%乙醇洗脱,收集乙醇洗脱液,回收乙醇再经Al2O3柱进行再脱色过程,用水洗至无色,浓缩得总皂苷。结果表明D101大孔吸附树脂纯化绞股蓝中总皂苷,用70%乙醇洗脱所得总皂苷含量最高,纯化率高达91.67%。绞股蓝中皂苷含量明显高于根部,在提取总皂苷的同时也能得到多糖蛋白。
The extraction, separation and purification of saponins from Gynostemma pentaphyllum were studied. By Gynostemma water precipitation alcohol precipitation, material to water ratio 1:10, concentrated 70% alcohol Shen, a polysaccharide protein. The supernatant was concentrated and recovered, respectively, on D101, LSA-21 macroporous resin adsorption, washed to colorless, with 30%, 50% ,, 70%, 90% ethanol elution, ethanol eluent was collected, ethanol recovery Then by the Al2O3 column decolorization process, washed with water to colorless, concentrated to give the total saponin. The results showed that D101 macroporous resin purified gypenosides purified with 70% ethanol, the highest content of total saponin, the purification rate as high as 91.67%. Gynostemma saponin content was significantly higher than the roots, in the extraction of total saponins can also get polysaccharide protein.