论文部分内容阅读
Objective To construct a recombinant bacillus Calmette-Guérin( BCG) vaccines based on different tan-dem repeats of MUC1 and GM-CSF,rBCG-MVNTR1 /4 / 8-CSF,and to observe the ability of three recombinant BCG vaccines in the inhibition of breast cancer. Methods After MUC1 variable-number tandem repeats ( MVN-TR1 /4 /8) were cloned in a stepwise manner,the E. coli-Mycobacteria shuttle expression vector pDE22-MVN-TR1 /4 /8-CSF were constructed by fusing MVNTR1 /4 /8 and GM-CSF,and then used to transform competent BCG
Objective To construct a recombinant bacillus Calmette-Guérin (BCG) vaccines based on different tan-dem repeats of MUC1 and GM-CSF, rBCG-MVNTR1 / 4/8-CSF, and to observe the ability of three recombinant BCG vaccines in the inhibition of breast cancer. Methods After MUC1 variable-number tandem repeats (MVN-TR1 / 4/8) were cloned in a stepwise manner, the E. coli- Mycobacteria shuttle expression vector pDE22- MVN- TR1 / 4 / 8- CSF were constructed by fusing MVNTR1 / 4/8 and GM-CSF, and then used to transform competent BCG