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引言自从Furuhashi和Yatazawa(1964)首先应用水稻茎节诱导愈伤组织获得成功以来,Nishi等(1968)和Yatazawa等(1967、1968)用根,Yamada等(1967)用根和茎节,Maeda(1965、1967、1969、1971)用幼苗和胚等,Wu和Li(1971)用叶鞘,Nakano等(1974、1975)用未成熟的胚乳以及Inoue和Maeda(1976)用种子或幼苗,也都先后获得了愈伤组织。而将水稻愈伤组织首次培养成苗者则为Niohi等(1968)。但迄今为止,在水稻中还未见有利用茎尖培养成苗的报导(Reinert等1977;Yamada,1977),这可能是由于水稻茎尖不易诱导形成愈伤组织之故(Inoue和Maeda,1976)。本报导系利用水稻的茎尖组织进行培
INTRODUCTION Nishi et al. (1968) and Yatazawa et al. (1967, 1968) used roots, Yamada et al. (1967) with roots and stems, Maeda ( 1965, 1967, 1969, 1971) with leaf sheaths for seedlings and embryos, Wu and Li (1971), immature endosperm by Nakano et al. (1974, 1975) and seeds or seedlings from Inoue and Maeda Callus was obtained. Niohi et al (1968) were the first to develop callus from rice calluses. However, up to now, there has been no report on the use of shoot tips for culture of seedlings in rice (Reinert et al. 1977; Yamada, 1977), probably due to the inability of rice shoot tips to induce callus formation (Inoue and Maeda 1976 ). This report uses the tip tissue of rice for cultivation