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以合成的前S2肽免疫兔获得抗血清。分别以抗前S2抗体和聚合人血清白蛋白作试剂,用固相放免法检测前S2和聚合人血清白蛋白结合活性。在HBsAg(-)的急性肝炎和各类慢性肝病病人中均未检出。在经肝活检诊断的慢性乙型活动性肝炎中,两者的检出率在病情增重期显著高于缓解期,亦高于肝组织正常的无症状HBV携带者。在HBeAg(+)组中两者的检出率均显著高于抗HBe(+)组。提示二者与宿主免疫应答或肝组织炎症活动有关。在上述检测中,聚合人血清白蛋白结合活性与前S2并不相同,后者的检出率仅为前者的56.7%。
Antisera were obtained by immunizing rabbits with the synthetic preS2 peptide. Anti-pre-S2 antibody and polymerized human serum albumin were used as reagents respectively to detect the binding activity of pre-S2 and polymerized human serum albumin by solid-phase radioimmunoassay. In HBsAg (-) of acute hepatitis and various types of patients with chronic liver disease were not detected. Detection of liver biopsy in patients with chronic hepatitis B, the detection rate of both in the condition of weight gain was significantly higher than the remission, also higher than the normal liver tissue of asymptomatic HBV carriers. The detection rate of both in HBeAg (+) group was significantly higher than that in anti-HBe (+) group. Suggesting that both the host immune response or inflammation of liver tissue activity. In the above test, the polymerization of human serum albumin binding activity and the former S2 are not the same, the latter detection rate was only 56.7% of the former.