A bimorph deformable mirror (DM) with a large stroke of more than 30 μm using 35 actuators is presented and characterized for an adaptive optics (AO) confocal scanning laser ophthalmoscope application. Facilitated with a Shack–Hartmann wavefront sensor, the bimorph DM-based AO operates closed-loop AO corrections for human eyes and reduces wavefront aberrations in most eyes to below 0.1 μm rms. Results from living eyes, including one exhibiting ～5D of myopia and ～2D of astigmatism along with notable high-order aberrations, reveal a practical efficient aberration correction and demonstrate a great benefit for retina imaging, including improving resolution, increasing brightness, and enhancing the contrast of images.