目的 观察高糖环境对人真皮微血管内皮细胞(HDMEC)功能的影响,探讨清除自由基对高糖所致HDMEC损伤的保护作用。方法 建立高糖环境下HDMEC培养的体外模型,并加入自由基清除剂MCI-186予以干扰,同时检测细胞的增殖、形态、黏附等生物学行为。结果 高糖环境可影响内皮细胞的增殖,改变其特异性形态,增加H2O2诱导的细胞毒性。MCI-186可缓解高糖所致的内皮细胞增殖受抑,降低内皮细胞表面黏附性,下调高糖诱导的细胞凋亡。结论 控制血糖的同时清除有害自由基,可能对糖尿病合并难愈创面的治疗起到积极作用。 Objective To observe the effects of high glucose on the function of human dermal microvascular endothelial cells (HDMECs) and to explore the protective effect of scavenging free radicals on HDMEC induced by high glucose. Methods The in vitro model of HDMEC cultured in high glucose environment was established, and the free radical scavenger MCI-186 was added to interfere with it. Meanwhile, the biological behaviors such as proliferation, morphology and adhesion were detected. Results High glucose environment could affect the proliferation of endothelial cells, change their specific morphology and increase the cytotoxicity induced by H2O2. MCI-186 can alleviate the inhibitory effect of high glucose on endothelial cell proliferation, reduce the adhesion of endothelial cells, and down-regulate the apoptosis induced by high glucose. Conclusion The control of blood sugar while removing harmful free radicals may play a positive role in the treatment of diabetic refractory wounds.