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对不同程度人工老化的辣椒种子芽期过氧化物酶同工酶和细胞色素氧化酶同工酶的谱带动态变化进行电泳分析表明，不同活力４的种子芽期细胞色素氧化酶同工酶酶带数呈由少到多最后趋向稳定的变化模式，低活力种子与高活力种子相比，谱带的出现有延迟现象；过氧化物酶同工酶酶带由于阴极第３条带的隐现呈波动增加的变化模式，但到第１１天和第１４天不同活力种子酶带相同。活力相近的种子酶带数变化相近，活力相差较大的种子酶带变化差异很大。因此，在种子纯度鉴定工作中，必须先对受试种子和标准种子作发芽试验，对所用的同工酶进行酶带动态变化分析，在此基础上，对于与标准种子活力相近的种子批可在芽期任一时间与标准种子同时电泳判定纯杂，对与标准种子活力相差很大的种子，则宜在发芽后期的谱带相对一致期内鉴定，才能提高鉴定结果的准确度。
The dynamic changes of the bands of peroxidase isozymes and cytochrome oxidase isoenzymes of artificial aging pepper seeds at the budding stage were analyzed by electrophoresis. The results showed that the activities of cytochrome oxidase isozymes The number of bands decreased from the last to the last, and the last tended to change steadily. Compared with the high-vigor seeds, there was a delay in the appearance of bands in the low-vigor seed. Peroxidase isozymes Fluctuations increased the pattern of change, but by the 11th and 14th days the different vigor seed bands were the same. Vigorously similar seed band changes in the number of similar vitality difference between the larger differences in the enzyme band changes. Therefore, in the seed purity identification work, we must first test seed and standard seed germination test, the isozyme used in the dynamic changes of the enzyme band, on this basis, with the standard seed vigor similar seed batch can be In the bud at any time with the standard seed electrophoresis determination of pure hybrid, and the standard seed vigor greatly different seeds, it is appropriate in the late germination of the band of the same period of identification in order to improve the accuracy of the identification results.