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目的探讨男性不育患者与Y染色体无精子症因子(AZF)微缺失的关系。方法采用聚合酶链反应技术对原发无精子症与少精子症患者Y染色体上AZF基因(STS)上的3个位点SY84、SY127、SY254及ZFX/ZFY基因进行检测。结果 45例无精子症与少精子症患者中共检出Y染色体微缺失9例,缺失率为20.0%。其中28例无精症患者中发现6例(21.43%),17例少精症患者中发现3例(17.65%)。结论 AZF缺失是原发无精子症和少精子症引起男性不育的重要遗传学原因之一。
Objective To investigate the relationship between male infertility and Y chromosome azoospermia factor (AZF) microdeletions. Methods The SY84, SY127, SY254 and ZFX / ZFY genes of AZF gene (STS) on Y chromosome of primary azoospermia and oligospermia were detected by polymerase chain reaction (PCR). Results Ninety-five patients with Y-chromosome microdeletions were detected in 45 patients with azoospermia and oligospermia, the deletion rate was 20.0%. Among 28 patients with azoospermia, 6 (21.43%) were found, and 3 (17.65%) were found in 17 patients with oligozoospermia. Conclusion AZF deletion is one of the important genetic causes of male infertility caused by primary azoospermia and oligospermia.