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目的探讨番茄红素对持续酒精摄入所致的酒精性肝损伤的拮抗作用。方法 100只健康成年SPF级昆明雄性小鼠随机分为5组:对照组、模型组和低、中、高剂量番茄红素组,每组20只。低、中、高剂量番茄红素组小鼠经口灌胃浓度分别为0.33、0.67和2.00 g/L的番茄红素玉米油混悬液,对照组与模型组灌胃等体积的玉米油,容量为0.2 ml/10 g,1次/d,连续30 d。第31 d始,给予上述试剂3 h后,对照组灌胃等量蒸馏水,其余4组灌胃56°白酒0.12 ml/10 g;1次/d,连续8 d。末次灌胃、禁食16 h后,测定血清中甘油三酯(TG)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、蛋白质羰基,肝脏中丙二醛(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)含量,同时计算死亡率、累积存活时间。结果与对照组比较,模型组血清中TG、ALT、AST、蛋白质羰基和肝脏中MDA含量增加,差异有统计学意义(P<0.01)。与模型组比较,中、高剂量番茄红素组小鼠死亡率降低,差异有统计学意义(P<0.05),存活时间延长;同时,肝脏中SOD、GSH-Px含量均高于模型组,差异有统计学意义(P<0.01),而血清中TG、ALT、AST、蛋白质羰基和肝脏中MDA含量降低,差异有统计学意义(P<0.05,P<0.01)。随着番茄红素灌胃剂量升高,小鼠血清中TG、ALT、AST、蛋白质羧基和肝脏中MDA含量呈下降趋势,而SOD、GSH-Px含量呈上升趋势。结论番茄红素对小鼠酒精性肝损伤具有一定的拮抗作用。
Objective To investigate the antagonistic effect of lycopene on alcoholic liver injury caused by continuous alcohol intake. Methods 100 healthy adult SPF Kunming male mice were randomly divided into 5 groups: control group, model group and low, medium and high dose lycopene group, 20 rats in each group. Low, medium and high doses of lycopene mice were orally fed with lycopene corn oil suspensions at the concentrations of 0.33, 0.67 and 2.00 g / L respectively. The control group and the model group were given the same volume of corn oil, Capacity of 0.2 ml / 10 g, 1 time / d, continuous 30 d. At the 31st day, the rats in the control group were given distilled water in the same amount for 3 hours and the other four groups were given 0.12 ml / 10 g of 56 ° liquor once per day for 8 days. After the last gavage and fasting for 16 h, the levels of triglyceride (TG), alanine aminotransferase (ALT), aspartate aminotransferase (AST), protein carbonyls, malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were measured. The mortality and cumulative survival time were also calculated. Results Compared with the control group, the levels of TG, ALT, AST, protein carbonyls and the content of MDA in the liver of model group increased significantly (P <0.01). Compared with the model group, the mortality of mice in middle and high dose of lycopene group was significantly lower (P <0.05) and the survival time was prolonged. At the same time, the content of SOD and GSH-Px in liver was higher than model group, (P <0.01). However, the content of TG, ALT, AST, protein carbonyl and the content of MDA in the liver decreased in the serum (P <0.05, P <0.01). With the increase of dosages of lycopene, the levels of MDA, TG, ALT, AST, protein carboxyl and liver in mice decreased, but the contents of SOD and GSH-Px increased. Conclusion Lycopene has antagonistic effect on alcoholic liver injury in mice.