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胶体金是近年来发展起来的一种新的免疫组化标记物,在各种酶类、放射性同位素、铁蛋白等标记物被采用的同时,胶体金标记法正越来越受到重视.胶体金标记的概述胶体金标记技术的应用仅十余年,十多年来,它的应用从电镜到光镜;从细胞表面物质的定位到细胞内抗原的定位,从单标记到多标记,从标记物的定位、定性研究到定量研究都取得了很大的进展。胶体金标记的原理是:氯化金水溶液在还原剂的作用下形成聚合的金颗粒,颗粒与颗粒间因静电
Colloidal gold is a new immunohistochemical marker developed in recent years. Colloidal gold labeling is gaining more and more attention when various enzymes, radioisotopes, ferritin and other markers are used. The application of colloidal gold labeling technology only ten years, its application from electron microscopy to light microscopy for more than 10 years; from the cell surface material localization to intracellular antigen localization, from single to multiple markers, from markers Material positioning, qualitative research to quantitative research have made great progress. The principle of colloidal gold labeling is: gold chloride aqueous solution under the action of a reducing agent to form polymerized gold particles, particles and particles due to static electricity