目的:研究雷公藤红素与大鼠、比格犬和人的血浆蛋白结合率。方法:采用血浆平衡透析法,以高效液相色谱法对透析内液与外液中雷公藤红素的含量进行测定,计算雷公藤红素与大鼠、比格犬和人的血浆蛋白结合率。结果:雷公藤红素低中高(0.25,0.5,1.0μg.mL-1)3个浓度的大鼠血浆蛋白结合率分别为(78.5±1.6)%,(77.3±2.2)%,(76.4±2.9)%,比格犬血浆蛋白结合率分别为(69.6±4.7)%,(71.1±3.4)%,(68.0±2.1)%,人血浆蛋白结合率分别为(85.6±2.7)%,(84.0±4.7)%,(83.1±2.8)%。结论:雷公藤红素与血浆蛋白具有中等强度的结合,其中与人血浆中的蛋白结合较强,且人>大鼠>比格犬。 Objective: To study the plasma protein binding rate of tripterine to rats, beagles and human. Methods: The plasma dialysis method was used to determine the content of tripterine in the dialysis solution and the solution by HPLC. The plasma protein binding rate of tripterine to rats, beagle dogs and human was calculated . Results: The plasma protein binding rates of triplotrazine low, medium, high (0.25,0.5,1.0μg.mL-1) were (78.5 ± 1.6)%, (77.3 ± 2.2)%, (76.4 ± 2.9) ), And the plasma protein binding rate of beagle dogs were (69.6 ± 4.7)%, (71.1 ± 3.4)% and (68.0 ± 2.1)%, and the plasma protein binding rates were (85.6 ± 2.7) 4.7%, (83.1 ± 2.8)%. Conclusion: Tripterine has a moderate binding to plasma proteins, which bind strongly to human plasma proteins, and human> rat> beagle dogs.