为建立一套适于凹叶厚朴幼叶的双向电泳(2-DE)体系,以凹叶厚朴幼叶为材料,对蛋白提取方法、上样量、聚焦程序优化,试验结果表明,TCA/丙酮+Tris-酚法提取蛋白干粉最佳,具有产量高、污染低、背景浅特点;选用17 cm pH5~8NL IPG预制胶条、15μg上样量、聚焦程序Ⅱ的2-DE电泳为最适凹叶厚朴蛋白双向电泳体系。该体系为后续凹叶厚朴分子机理研究奠定基础。 In order to establish a set of two-dimensional gel electrophoresis (2-DE) system for young leaves of Magnolia biloba, the methods of protein extraction, sample loading and focusing were optimized using young leaves of Magnolia biloba. The results showed that TCA / acetone + Tris - Phenol method to extract the best dry powder protein, with high yield, low pollution, shallow background characteristics; selection 17cm pH5 ~ 8NL IPG preformed strips, 15μg sample volume, focusing program Ⅱ 2-DE electrophoresis as the optimal pachyrhizus Two-dimensional electrophoresis system. This system lays the foundation for the molecular mechanism research of the next Euonymus japonicus.