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目的:探讨人重组肝细胞生长因子(rhHGF)对结肠癌细胞SW480和SW620中3种内皮生长因子VEGF-A、VEGF-C和VEGF-D表达的影响。方法:实验分对照组(5%胎牛血清)和HGF组(80 ng/mL的HGF)。采用MTT法分析HGF对肿瘤细胞增殖的作用,流式细胞术检测HGF对细胞周期的影响。48 h后进行蛋白质印迹法检测,并对信号强度进行相对定量分析。结果:HGF能促进SW480和SW620增殖。流式细胞术结果显示,与对照组相比,SW480的HGF组中的G0/G1期细胞减少,S期细胞增多,G2/M期细胞增多,但HGF对SW620细胞周期的影响不明显。SW480细胞HGF组的VEGF-A表达量是对照组的1.59倍,SW620细胞HGF组的VEGF-A表达量是对照组的2.08倍(P<0.01);SW480细胞HGF组的VEGF-C表达量是对照组的1.37倍,SW620细胞HGF组的VEGF-C表达量是对照组的1.27倍(P<0.01);SW480细胞HGF组的VEGF-D表达量是对照组的1.46倍,SW620细胞HGF组的VEGF-D表达量是对照组的1.38倍(P<0.01)。结论:HGF通过上调结直肠癌细胞VEGF-A、VEGF-C和VEGF-D的表达而促进肿瘤血管和淋巴管的新生,可能是潜在的结直肠癌治疗的新靶点。
Objective: To investigate the effect of recombinant human hepatocyte growth factor (rhHGF) on the expression of VEGF-A, VEGF-C and VEGF-D in three kinds of human colon cancer cells SW480 and SW620. Methods: The experiment was divided into control group (5% fetal bovine serum) and HGF group (80 ng / mL HGF). The effect of HGF on tumor cell proliferation was analyzed by MTT assay. The effect of HGF on cell cycle was detected by flow cytometry. After 48 h Western blotting was performed, and the relative quantitative analysis of signal intensity. Results: HGF can promote the proliferation of SW480 and SW620. Flow cytometry showed that compared with the control group, the number of G0 / G1 phase cells, the S phase cells and the G2 / M phase cells increased in SW480 HGF group, but the effect of HGF on SW620 cell cycle was not obvious. The expression of VEGF-A in HGF group was 1.59-fold higher than that in SW480 cells, and the expression of VEGF-A in HGF group was 2.08-fold (P <0.01) in SW480 cells. The expression of VEGF-C in SW480 cells was The level of VEGF-C in HGF group was 1.27 times that of the control group (P <0.01), and the level of VEGF-D in SW480 cells was 1.46 times that of the control group The expression of VEGF-D was 1.38 times that of the control group (P <0.01). CONCLUSION: HGF promotes neovascularization of tumor vessels and lymphatic vessels by up-regulating the expression of VEGF-A, VEGF-C and VEGF-D in colorectal cancer cells, which may be a new target of potential colorectal cancer therapy.